Serrano K, Levin E, Chen D, Hansen A, Turner T R, Kurach J, Reidel A, Boecker W F, Acker J P, Devine D V
Canadian Blood Services' Centre for Innovation, The Centre for Blood Research, University of British Columbia, Vancouver, BC, Canada.
Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.
Vox Sang. 2016 Apr;110(3):227-35. doi: 10.1111/vox.12355. Epub 2015 Dec 8.
Di-2-ethylhexyl phthalate (DEHP) is a blood bag plasticizer. It is also a toxin, raising concerns for vulnerable populations, for example, neonates and infants. Here, the in vitro quality of red cell concentrates (RCC) stored in paediatric bags formulated with alternative plasticizers to DEHP was compared.
RCC were pooled and split into polyvinylchloride (PVC)/DEHP, PVC/1,2-cyclohexanedicarboxylic acid diisononyl ester (DINCH) or PVC/butyryl trihexyl citrate (BTHC) bags. Quality was assessed on storage days 5, 21, 35 and 43.
Metabolism differed among the bags: pCO2 levels were lowest and pO2 were highest in BTHC bags. Glucose consumption and lactate production suggested higher metabolic rates in BTHC bags. ATP levels were best maintained in DINCH bags (day 43 mean level: 2·86 ± 0·29 μmol/g Hb). RCC in BTHC bags had the greatest potassium release (54·6 ± 3·0 mm on day 43). From day 21, haemolysis was higher in BTHC bags (P < 0·01) and by day 43 had exceeded 0·8% (0·85 ± 0·10%). RCC in BTHC bags showed more microparticle formation than RCC in DEHP or DINCH bags.
The results suggest that the BTHC formulation used was detrimental to RBC quality. DINCH bags could be a viable alternative to DEHP: they outperformed DEHP bags energetically, with better maintenance of ATP levels.
邻苯二甲酸二（2-乙基己基）酯（DEHP）是一种血袋增塑剂。它也是一种毒素，这引发了对弱势群体（如新生儿和婴儿）的担忧。在此，比较了用替代DEHP的增塑剂配制的儿科血袋中储存的红细胞浓缩液（RCC）的体外质量。
将RCC汇集并分成聚氯乙烯（PVC）/DEHP、PVC/1,2-环己烷二羧酸二异壬酯（DINCH）或PVC/柠檬酸三己酯（BTHC）血袋。在储存第5、21、35和43天评估质量。
各血袋之间的代谢情况不同：BTHC血袋中的pCO2水平最低，pO2最高。葡萄糖消耗和乳酸生成表明BTHC血袋中的代谢率更高。DINCH血袋中ATP水平维持得最好（第43天平均水平：2·86±0·29μmol/g血红蛋白）。BTHC血袋中的RCC钾释放量最大（第43天为54·6±3·0mmol）。从第21天起，BTHC血袋中的溶血率更高（P<0·01），到第43天时超过了0·8%（0·85±0·10%）。BTHC血袋中的RCC比DEHP或DINCH血袋中的RCC形成更多的微粒。
结果表明所用的BTHC配方对红细胞质量有害。DINCH血袋可能是DEHP的可行替代品：它们在维持ATP水平方面比DEHP血袋表现更好。
