Sajjanar Basavaraj, Saxena Shikha, Bisht Deepika, Singh Arvind Kumar, Manjunatha Reddy G B, Singh Rajendra, Singh R P, Kumar Satish
Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Bareilly 243122, Uttar Pradesh, India.
National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064, Karnataka, India.
Neuropeptides. 2016 Jun;57:59-64. doi: 10.1016/j.npep.2015.11.090. Epub 2015 Nov 30.
Rabies virus (RABV) is neurotropic and causes acute progressive encephalitis. Herein, we report the interaction of nAChRα1-subunit peptides with RABV and the effect of these peptides on RABV infection in cultured neuronal cells. Peptide sequences derived from torpedo, bovine, human and rats were synthesized and studied for their interactions with RABV using virus capture ELISA and peptide immunofluorescence. The results showed specific binding of the nAChRα1-subunit peptides to the RABV. In the virus adsorption assay, these peptides were found to inhibit the attachment of the RABV to the neuronal cells. The nAChRα1-subunit peptides inhibited the RABV infection and reduced viral gene expression in the cultured neuroblastoma (N2A) cells. Torpedo peptide sequence (T-32) had highest antiviral effect (IC50=14±3.01μM) compared to the other peptides studied. The results of the study indicated that nAChRα1-subunit peptides may act as receptor decoy molecules and inhibit the binding of virus to the native host cell receptors and hence may reduce viral infection.
狂犬病病毒（RABV）具有嗜神经性，可引起急性进行性脑炎。在此，我们报告了烟碱型乙酰胆碱受体α1亚基肽与RABV的相互作用以及这些肽对培养的神经元细胞中RABV感染的影响。合成了源自电鳐、牛、人和大鼠的肽序列，并使用病毒捕获ELISA和肽免疫荧光研究了它们与RABV的相互作用。结果显示烟碱型乙酰胆碱受体α1亚基肽与RABV存在特异性结合。在病毒吸附试验中，发现这些肽可抑制RABV与神经元细胞的附着。烟碱型乙酰胆碱受体α1亚基肽抑制了培养的神经母细胞瘤（N2A）细胞中的RABV感染并降低了病毒基因表达。与所研究的其他肽相比，电鳐肽序列（T-32）具有最高的抗病毒效果（IC50=14±3.01μM）。该研究结果表明，烟碱型乙酰胆碱受体α1亚基肽可能作为受体诱饵分子，抑制病毒与天然宿主细胞受体的结合，从而可能减少病毒感染。
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