采用超高效液相色谱-串联质谱法（UPLC-MS/MS）对血浆中口服抗凝剂达比加群、利伐沙班、阿哌沙班和华法林进行定量分析。

Quantitation of the Oral Anticoagulants Dabigatran, Rivaroxaban, Apixaban, and Warfarin in Plasma Using Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry (UPLC-MS/MS).

作者信息

Noguez Jaime H, Ritchie James C

机构信息

Department of Pathology, University Hospitals Case Medical Center, 11100 Euclid Avenue, PTH 5077, Cleveland, OH, 44106, USA.

Department of Pathology and Laboratory Medicine, Emory University Hospital, Atlanta, GA, USA.

出版信息

Methods Mol Biol. 2016;1383:21-7. doi: 10.1007/978-1-4939-3252-8_3.

DOI:10.1007/978-1-4939-3252-8_3

PMID:26660170

Abstract

This chapter describes a method to measure the oral anticoagulants dabigatran, rivaroxaban, apixaban, and warfarin in plasma samples using ultra-performance liquid chromatography combined with tandem mass spectrometry (UPLC-MS/MS). The instrument is operated in multiple reaction monitoring (MRM) mode with an electrospray ionization (ESI) source in positive ionization mode. Samples are extracted with a 90:10 methanol/0.1 N hydrochloric acid solution containing stable isotope-labeled internal standards for each analyte. After centrifugation the supernatant is transferred to a mass spectrometry vial, injected onto the UPLC-ESI-MS/MS, and quantified using an eight-point calibration curve.

摘要

本章介绍了一种使用超高效液相色谱-串联质谱法（UPLC-MS/MS）测定血浆样本中口服抗凝剂达比加群、利伐沙班、阿哌沙班和华法林的方法。该仪器在多反应监测（MRM）模式下运行，采用电喷雾电离（ESI）源，处于正离子模式。样本用含有每种分析物的稳定同位素标记内标的90:10甲醇/0.1N盐酸溶液萃取。离心后，将上清液转移至质谱小瓶中，注入UPLC-ESI-MS/MS，并使用八点校准曲线进行定量。