从玛雅药用植物 Cestrum schlechtendahlii G. Don（茄科）中提取的抗真菌皂苷。

Antifungal Saponins from the Maya Medicinal Plant Cestrum schlechtendahlii G. Don (Solanaceae).

机构信息

Laboratory for Analysis of Natural and Synthetic Environmental Toxins (LANSET), Department of Biology, University of Ottawa, Ottawa, Canada.

Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Carretera Antigua a Coatepec, Xalapa, Veracruz, Mexico.

出版信息

Phytother Res. 2016 Mar;30(3):439-46. doi: 10.1002/ptr.5545. Epub 2015 Dec 15.

DOI:10.1002/ptr.5545

PMID:26666462

Abstract

Bioassay-guided fractionation of the crude extract (80% EtOH) of the leaves of Cestrum schlechtendahlii, a plant used by Q'eqchi' Maya healers for treatment of athlete's foot, resulted in the isolation and identification of two spirostanol saponins (1 and 2). Structure elucidation by MS, 1D-NMR, and 2D-NMR spectroscopic methods identified them to be the known saponin (25R)-1β,2α-dihydroxy-5α-spirostan-3-β-yl-O-α-L-rhamnopyranosyl-(1 → 2)-β-D-galactopyranoside (1) and new saponin (25R)-1β,2α-dihydroxy-5α-spirostan-3-β-yl-O-β-D-galactopyranoside (2). While 2 showed little or no antifungal activity at the highest concentration tested, 1 inhibited growth of Saccharomyces cerevisiae (minimum inhibitory concentration (MIC) of 15-25 μM), Candida albicans, Cryptococcus neoformans, and Fusarium graminearum (MIC of 132-198 μM).

摘要

生物测定指导下对用于治疗足癣的 Q'eqchi' 玛雅治疗师所使用的希氏离瓣苣苔叶的 80%乙醇粗提物进行分段，得到两种螺旋甾烷醇皂苷（1 和 2）的分离和鉴定。通过 MS、1D-NMR 和 2D-NMR 光谱方法进行结构阐明，鉴定它们为已知皂苷（25R）-1β，2α-二羟基-5α-螺旋甾烷-3-β-基-O-α-L-鼠李吡喃糖苷基-（1 → 2）-β-D-半乳糖吡喃糖苷（1）和新皂苷（25R）-1β，2α-二羟基-5α-螺旋甾烷-3-β-基-O-β-D-半乳糖吡喃糖苷（2）。虽然 2 在测试的最高浓度下几乎没有或没有抗真菌活性，但 1 抑制酿酒酵母（最小抑菌浓度（MIC）为 15-25 μM）、白色念珠菌、新型隐球菌和禾谷镰刀菌的生长（MIC 为 132-198 μM）。