微小RNA-411抑制人软骨细胞中基质金属蛋白酶13的表达。
MicroRNA-411 inhibited matrix metalloproteinase 13 expression in human chondrocytes.
作者信息
Wang Guodong, Zhang Yuanmin, Zhao Xiaowei, Meng Chunyang, Ma Longfei, Kong Ying
机构信息
Department of Orthopaedics, Affiliated Hospital of Jining Medical University Jining 272029, China.
出版信息
Am J Transl Res. 2015 Oct 15;7(10):2000-6. eCollection 2015.
Osteoarthritis (OA) is the most common joint degenerative disease affecting the joint structure, leading to loss of joint function and tissue destruction. Recent studies have demonstrated that miRNAs are involved in many pathological conditions, including OA. The study was to investigate the role of miR-411 in the pathogenesis of OA. The expression of miR-411 was downregulated in OA cartilage compared with in normal cartilage. Conversely, the expression of MMP-13 was upregulated in OA cartilage compared with in normal cartilage. IL-1β treatment repressed miR-411 expression in chondrocytes. Moreover, we identified MMP-13 as a direct target gene of miR-411 in chondrocytes and overexpression of miR-411 inhibited the MMP-13 expression. Furthermore, overexpression of miR-411 increased the expression of type II collagen and type IV collagen expression in chondrocytes. MiR-411 is a crucial regulator of MMP-13 in chondrocytes and may response to the development of OA.
骨关节炎（OA）是影响关节结构的最常见的关节退行性疾病，会导致关节功能丧失和组织破坏。最近的研究表明，微小RNA（miRNAs）参与包括OA在内的许多病理状况。本研究旨在探讨miR-411在OA发病机制中的作用。与正常软骨相比，OA软骨中miR-411的表达下调。相反，与正常软骨相比，OA软骨中基质金属蛋白酶-13（MMP-13）的表达上调。白细胞介素-1β（IL-1β）处理可抑制软骨细胞中miR-411的表达。此外，我们确定MMP-13是软骨细胞中miR-411的直接靶基因，miR-411的过表达抑制了MMP-13的表达。此外，miR-411的过表达增加了软骨细胞中II型胶原蛋白和IV型胶原蛋白的表达。MiR-411是软骨细胞中MMP-13的关键调节因子，可能与OA的发展有关。
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