印度南部引起人类角膜真菌病的镰刀菌属机会致病菌的抗真菌药敏性及系统发育

Antifungal Susceptibility and Phylogeny of Opportunistic Members of the Genus Fusarium Causing Human Keratomycosis in South India.

作者信息

Hassan Anamangadan Shafeeq, Al-Hatmi Abdullah M S, Shobana Coimbatore Subramanian, van Diepeningen Anne D, Kredics László, Vágvölgyi Csaba, Homa Mónika, Meis Jacques F, de Hoog G Sybren, Narendran Venkatapathy, Manikandan Palanisamy

机构信息

Department of Microbiology, Dr. G.R. Damodaran College of Science, Coimbatore, India.

CBS-KNAW Fungal Biodiversity Centre, Utrecht, the Netherlands Institute of Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, the Netherlands Directorate General of Health Services, Ministry of Health, Ibri Hospital, Ibri, Oman.

出版信息

Med Mycol. 2016 Mar;54(3):287-94. doi: 10.1093/mmy/myv105. Epub 2015 Dec 24.

DOI:10.1093/mmy/myv105

PMID:26705832

Abstract

Fusarium species are reported frequently as the most common causative agents of fungal keratitis in tropical countries such as India. Sixty-five fusaria isolated from patients were subjected to multilocus DNA sequencing to characterize the spectrum of the species associated with keratitis infections in India. Susceptibilities of these fusaria to ten antifungals were determined in vitro by the broth microdilution method. An impressive phylogenetic diversity of fusaria was reflected in susceptibilities differing at species level. Typing results revealed that the isolates were distributed among species in the species complexes (SCs) of F. solani (FSSC; n = 54), F. oxysporum (FOSC; n = 1), F. fujikuroi (FFSC; n = 3), and F. dimerum (FDSC; n = 7). Amphotericin B, voriconazole, and clotrimazole proved to be the most effective drugs, followed by econazole.

摘要

在印度等热带国家，镰刀菌属经常被报道为真菌性角膜炎最常见的病原体。从患者身上分离出的65株镰刀菌进行多位点DNA测序，以确定与印度角膜炎感染相关的菌种谱。通过肉汤微量稀释法在体外测定这些镰刀菌对十种抗真菌药物的敏感性。镰刀菌在物种水平上的敏感性差异反映出其令人印象深刻的系统发育多样性。分型结果显示，分离株分布在茄病镰刀菌（FSSC；n = 54）、尖孢镰刀菌（FOSC；n = 1）、藤仓镰刀菌（FFSC；n = 3）和双隔镰刀菌（FDSC；n = 7）的物种复合体（SCs）中的各个物种之间。两性霉素B、伏立康唑和克霉唑被证明是最有效的药物，其次是益康唑。