组织因子在喉癌患者的癌旁组织中强烈表达。
Tissue factor is strongly expressed in pericarcinomatous tissue in patients with laryngeal carcinoma.
作者信息
Wang Bin, Xiong Sufang, Hua Qingquan, Chen Chen, Liao Hua, Chen Liu, Yao Weiqi, Wu Dongcheng, Tao Zezhang
机构信息
Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University Wuhan 430060, China.
The Fifth Hospital in Wuhan Wuhan 430000, China.
出版信息
Int J Clin Exp Pathol. 2015 Oct 1;8(10):13719-24. eCollection 2015.
OBJECTIVE
This study aimed to understand the relationship between tissue factor (TF) and laryngeal carcinoma.
METHODS
Differences in TF expression between pericarcinomatous and carcinomatous tissues were studied in patients with laryngeal carcinoma; the potential clinical significance of the observed differences is discussed. Immunohistochemical, western blot, and RT-PCR analyses were performed to assess the expression of TF at the protein and mRNA levels, and differences between pericarcinomatous and carcinomatous tissues in patients (n = 20) with laryngeal carcinoma were analyzed.
RESULTS
Expression of TF was significantly higher in pericarcinomatous tissues than in carcinomatous tissues (P < 0.01); furthermore, the intensity of TF mRNA expression was also significantly stronger in pericarcinomatous than in carcinomatous tissue (P < 0.001). Robust expression of TF was observed in pericarcinomatous tissues but not in carcinomatous tissues.
CONCLUSION
TF may contribute to the carcinogenesis and development of laryngeal carcinoma and may provide a marker for assessment of the degree of malignancy and the progression of laryngeal carcinoma. TF may also provide a new target for therapeutics for human head and neck cancer.
目的
本研究旨在了解组织因子（TF）与喉癌之间的关系。
方法
对喉癌患者癌旁组织和癌组织中TF表达的差异进行研究，并探讨所观察到的差异的潜在临床意义。进行免疫组织化学、蛋白质印迹和逆转录聚合酶链反应（RT-PCR）分析，以评估TF在蛋白质和mRNA水平的表达，并分析20例喉癌患者癌旁组织和癌组织之间的差异。
结果
TF在癌旁组织中的表达显著高于癌组织（P < 0.01）；此外，TF mRNA表达强度在癌旁组织中也显著强于癌组织（P < 0.001）。在癌旁组织中观察到TF的强烈表达，但在癌组织中未观察到。
结论
TF可能有助于喉癌的发生和发展，并可能为评估喉癌的恶性程度和进展提供一个标志物。TF也可能为人类头颈癌的治疗提供一个新的靶点。
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