Kitpreechavanich Vichien, Hayami Arisa, Talek Anfal, Chin Clament Fui Seung, Tashiro Yukihiro, Sakai Kenji
Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
J Biosci Bioeng. 2016 Jul;122(1):105-10. doi: 10.1016/j.jbiosc.2015.12.017. Epub 2016 Jan 23.
A unique method to produce highly optically-active l-lactic acid and soil amendments that promote plant growth from food waste was proposed. Three Bacillus strains Bacillus subtilis KBKU21, B. subtilis N3-9 and Bacillus coagulans T27, were used. Strain KBKU21 accumulated 36.9 g/L l-lactic acid with 95.7% optical activity and 98.2% l-lactic acid selectivity when fermented at 43°C for 84 h in a model kitchen refuse (MKR) medium. Residual precipitate fraction (anaerobically-fermented MKR (AFM) compost) analysis revealed 4.60%, 0.70% and 0.75% of nitrogen (as N), phosphorous (as P2O5), and potassium (as K2O), respectively. Additionally, the carbon to nitrogen ratio decreased from 13.3 to 10.6. AFM compost with KBKU21 promoted plant growth parameters, including leaf length, plant height and fresh weight of Brassica rapa (Komatsuna), than that by chemical fertilizers or commercial compost. The concept provides an incentive for the complete recycling of food waste, contributing towards a sustainable production system.
提出了一种从食物垃圾中生产高光学活性L-乳酸和促进植物生长的土壤改良剂的独特方法。使用了三种芽孢杆菌菌株，即枯草芽孢杆菌KBKU21、枯草芽孢杆菌N3-9和凝结芽孢杆菌T27。菌株KBKU21在模拟厨房垃圾（MKR）培养基中于43°C发酵84小时后，积累了36.9 g/L的L-乳酸，光学活性为95.7%，L-乳酸选择性为98.2%。对残留沉淀部分（厌氧发酵的MKR（AFM）堆肥）的分析表明，氮（以N计）、磷（以P2O5计）和钾（以K2O计）的含量分别为4.60%、0.70%和0.75%。此外，碳氮比从13.3降至10.6。与使用化肥或商业堆肥相比，含有KBKU21的AFM堆肥促进了油菜（小松菜）的植物生长参数，包括叶长、株高和鲜重。这一概念为食物垃圾的完全回收提供了动力，有助于建立可持续的生产系统。
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