衰减全反射傅里叶变换红外光谱和拉曼光谱法研究含抗肿瘤药物纳米载体系统的电穿孔介导透皮给药
ATR-FTIR and Raman spectroscopic investigation of the electroporation-mediated transdermal delivery of a nanocarrier system containing an antitumour drug.
作者信息
Balázs Boglárka, Sipos Péter, Danciu Corina, Avram Stefana, Soica Codruta, Dehelean Cristina, Varju Gábor, Erős Gábor, Budai-Szűcs Mária, Berkó Szilvia, Csányi Erzsébet
机构信息
Department of Pharmaceutical Technology, University of Szeged, Szeged, H-6720, Hungary; Gedeon Richter Plc., Budapest, H-1103, Hungary.
Department of Pharmaceutical Technology, University of Szeged, Szeged, H-6720, Hungary.
出版信息
Biomed Opt Express. 2015 Dec 8;7(1):67-78. doi: 10.1364/BOE.7.000067. eCollection 2016 Jan 1.
Abstract
The aim of the present work was the optimization of the transdermal delivery of a lyotropic liquid crystal genistein-based formulation (LLC-GEN). LLC was chosen as medium in view of the poor solubility of GEN in water. Membrane diffusion and penetration studies were carried out with a Franz diffusion cell, through a synthetic membrane in vitro, a chick chorioallantoic membrane ex ovo, and ex vivo excised human epidermis. Thereafter, LLC-GEN was combined with electroporation (EP) to enhance the transdermal drug delivery. The synergistic effect of EP was verified by in vivo ATR-FTIR and ex vivo Raman spectroscopy on hairless mouse skin.
摘要
本研究的目的是优化基于溶致液晶染料木黄酮的制剂（LLC-GEN）的透皮给药。鉴于染料木黄酮在水中的溶解度较差，选择了液晶作为介质。使用Franz扩散池，通过体外合成膜、鸡胚绒毛尿囊膜和离体切除的人表皮进行了膜扩散和渗透研究。此后，将LLC-GEN与电穿孔（EP）相结合以增强透皮给药。通过体内衰减全反射傅里叶变换红外光谱（ATR-FTIR）和体外拉曼光谱对无毛小鼠皮肤验证了电穿孔的协同作用。
相似文献
1
ATR-FTIR and Raman spectroscopic investigation of the electroporation-mediated transdermal delivery of a nanocarrier system containing an antitumour drug.
Biomed Opt Express. 2015 Dec 8;7(1):67-78. doi: 10.1364/BOE.7.000067. eCollection 2016 Jan 1.
2
Papaverine hydrochloride containing nanostructured lyotropic liquid crystal formulation as a potential drug delivery system for the treatment of erectile dysfunction.
Drug Des Devel Ther. 2018 Sep 12;12:2923-2931. doi: 10.2147/DDDT.S168218. eCollection 2018.
3
On the Structure and Transdermal Profile of Liquid Crystals Based on Phytantriol.
Curr Drug Deliv. 2018;15(10):1439-1448. doi: 10.2174/1567201815666180910142516.
4
Penetration monitoring of drugs and additives by ATR-FTIR spectroscopy/tape stripping and confocal Raman spectroscopy - A comparative study.
Eur J Pharm Biopharm. 2018 Sep;130:214-223. doi: 10.1016/j.ejpb.2018.07.007. Epub 2018 Jul 5.
5
The Effect of Electroporation of a Lyotroic Liquid Crystal Genistein-Based Formulation in the Recovery of Murine Melanoma Lesions.
Int J Mol Sci. 2015 Jul 8;16(7):15425-41. doi: 10.3390/ijms160715425.
6
The Effect of Prescription on the Framework of Lipid Matrix and In Vitro Properties.
Curr Drug Deliv. 2019;16(8):737-750. doi: 10.2174/1567201816666190620115403.
7
ATR-FTIR spectroscopy and spectroscopic imaging of solvent and permeant diffusion across model membranes.
Eur J Pharm Biopharm. 2010 Feb;74(2):413-9. doi: 10.1016/j.ejpb.2009.11.004. Epub 2009 Nov 12.
8
[Modelling of pecutaneous drug permeation and investigation of penetration enhancer effect].
Acta Pharm Hung. 2012;82(1):15-22.
9
Mechanism of the synergic effects of calcium chloride and electroporation on the in vitro enhanced skin permeation of drugs.
J Control Release. 2004 Mar 5;95(2):267-74. doi: 10.1016/j.jconrel.2003.12.014.
10
In vivo real-time monitoring system of electroporation mediated control of transdermal and topical drug delivery.
J Control Release. 2013 Dec 28;172(3):862-71. doi: 10.1016/j.jconrel.2013.09.030. Epub 2013 Oct 7.
引用本文的文献
1
Imaging and quantifying drug delivery in skin - Part 2: Fluorescence andvibrational spectroscopic imaging methods.
Adv Drug Deliv Rev. 2020 Jan 1;153:147-168. doi: 10.1016/j.addr.2020.03.003. Epub 2020 Mar 23.
2
Botanical Therapeutics: Phytochemical Screening and Biological Assessment of Chamomile, Parsley and Celery Extracts against A375 Human Melanoma and Dendritic Cells.
Int J Mol Sci. 2018 Nov 16;19(11):3624. doi: 10.3390/ijms19113624.
3
Quantitative two-photon microscopy imaging analysis of human skin to evaluate enhanced transdermal delivery by hybrid-type multi-lamellar nanostructure.
Biomed Opt Express. 2018 Jul 31;9(8):3974-3982. doi: 10.1364/BOE.9.003974. eCollection 2018 Aug 1.
4
Full depth measurement of tenofovir transport in rectal mucosa using confocal Raman spectroscopy and optical coherence tomography.
Drug Deliv Transl Res. 2018 Jun;8(3):843-852. doi: 10.1007/s13346-018-0495-7.
5
Controlled release of optimized electroporation enhances the transdermal efficiency of sinomenine hydrochloride for treating arthritis in vitro and in clinic.
Drug Des Devel Ther. 2017 Jun 15;11:1737-1752. doi: 10.2147/DDDT.S136313. eCollection 2017.
6
Fabrication of a Ti porous microneedle array by metal injection molding for transdermal drug delivery.
PLoS One. 2017 Feb 10;12(2):e0172043. doi: 10.1371/journal.pone.0172043. eCollection 2017.
本文引用的文献
1
The Effect of Electroporation of a Lyotroic Liquid Crystal Genistein-Based Formulation in the Recovery of Murine Melanoma Lesions.
Int J Mol Sci. 2015 Jul 8;16(7):15425-41. doi: 10.3390/ijms160715425.
2
A comparative study between human skin substitutes and normal human skin using Raman microspectroscopy.
Acta Biomater. 2014 Jun;10(6):2703-11. doi: 10.1016/j.actbio.2014.02.007. Epub 2014 Feb 12.
3
Effect of the isoflavone genistein on tumor size, metastasis potential and melanization in a B16 mouse model of murine melanoma.
Nat Prod Commun. 2013 Mar;8(3):343-6.
4
Infrared and Raman imaging spectroscopy of ex vivo skin.
Int J Cosmet Sci. 2013 Apr;35(2):125-35. doi: 10.1111/ics.12020. Epub 2012 Nov 29.
5
Localization and interaction of genistein with model membranes formed with dipalmitoylphosphatidylcholine (DPPC).
Biochim Biophys Acta. 2012 Jul;1818(7):1785-93. doi: 10.1016/j.bbamem.2012.03.020.
6
Ibuprofen penetration enhance by sucrose ester examined by ATR-FTIR in vivo.
Pharm Dev Technol. 2012 Jan-Feb;17(1):125-8. doi: 10.3109/10837450.2010.508076. Epub 2010 Aug 17.
7
In vivo infrared spectroscopy studies of alkanol effects on human skin.
Eur J Pharm Biopharm. 2008 Aug;69(3):1171-5. doi: 10.1016/j.ejpb.2008.02.006. Epub 2008 Feb 16.
8
The chick embryo and its chorioallantoic membrane (CAM) for the in vivo evaluation of drug delivery systems.
Adv Drug Deliv Rev. 2007 Sep 30;59(11):1162-76. doi: 10.1016/j.addr.2007.04.019. Epub 2007 Aug 16.
9
Physical characterization of the stratum corneum of an in vitro psoriatic skin model by ATR-FTIR and Raman spectroscopies.
Biochim Biophys Acta. 2007 Sep;1770(9):1317-23. doi: 10.1016/j.bbagen.2007.06.014. Epub 2007 Jul 12.
10
Delivery of ethinylestradiol from film forming polymeric solutions across human epidermis in vitro and in vivo in pigs.
J Control Release. 2007 Apr 2;118(2):196-203. doi: 10.1016/j.jconrel.2006.12.013. Epub 2006 Dec 22.
Zotero 插件