Subramani Baskar, Subbannagounder Sellamuthu, Palanivel Sekar, Ramanathanpullai Chithra, Sivalingam Sivakumar, Yakub Azhari, SadanandaRao Manjunath, Seenichamy Arivudainambi, Pandurangan Ashok Kumar, Tan Jun Jie, Ramasamy Rajesh
Nichi-Asia Life Sdn Bhd., 47810, Petaling Jaya, Selangor, Malaysia.
Bharathiyar University, Coimbatore, Tamil Nadu, India.
Cytotechnology. 2016 Oct;68(5):2061-73. doi: 10.1007/s10616-016-9946-5. Epub 2016 Jan 28.
Despite the surgical and other insertional interventions, the complete recuperation of myocardial disorders is still elusive due to the insufficiency of functioning myocardiocytes. Thus, the use of stem cells to regenerate the affected region of heart becomes a prime important. In line with this human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) have gained considerable interest due to their potential use for mesodermal cell based replacement therapy and tissue engineering. Since MSCs are harvested from various organs and anatomical locations of same organism, thus the cardiac regenerative potential of human cardiac-derived MSCs (hC-MSCs) and human umbilical cord Wharton's Jelly derived MSC (hUC-MSCs) were tested concurrently. At in vitro culture, both hUC-MSCs and hC-MSCs assumed spindle shape morphology with expression of typical MSC markers namely CD105, CD73, CD90 and CD44. Although, hUC-MSCs and hC-MSCs are identical in term of morphology and immunophenotype, yet hUC-MSCs harbored a higher cell growth as compared to the hC-MSCs. The inherent cardiac regenerative potential of both cells were further investigated with mRNA expression of ion channels. The RT-PCR results demonstrated that both MSCs were expressing a notable level of delayed rectifier-like K(+) current (I KDR ) ion channel, yet the relative expression level was considerably varied between hUC-MSCs and hC-MSCs that Kv1.1(39 ± 0.6 vs 31 ± 0.8), Kv2.1 (6 ± 0.2 vs 21 ± 0.12), Kv1.5 (7.4 ± 0.1 vs 6.8 ± 0.06) and Kv7.3 (27 ± 0.8 vs 13.8 ± 0.6). Similarly, the Ca2(+)-activated K(+) current (I KCa ) channel encoding gene, transient outward K(+) current (I to ) and TTX-sensitive transient inward sodium current (I Na.TTX ) encoding gene (Kv4.2, Kv4.3 and hNE-Na) expressions were detected in both groups as well. Despite the morphological and phenotypical similarity, the present study also confirms the existence of multiple functional ion channel currents IKDR, IKCa, Ito, and INa.TTX in undifferentiated hUC-MSCs as of hC-MSCs. Thus, the hUC-MSCs can be exploited as a potential candidate for future cardiac regeneration.
尽管进行了手术及其他植入干预，但由于功能性心肌细胞不足，心肌疾病仍难以完全康复。因此，利用干细胞再生心脏受影响区域变得至关重要。与此相符的是，人脐带间充质干细胞（hUC-MSCs）因其在中胚层细胞替代疗法和组织工程中的潜在应用而备受关注。由于间充质干细胞可从同一生物体的不同器官和解剖位置获取，因此同时测试了人心脏来源的间充质干细胞（hC-MSCs）和人脐带华通氏胶来源的间充质干细胞（hUC-MSCs）的心脏再生潜力。在体外培养时，hUC-MSCs和hC-MSCs均呈现纺锤形形态，并表达典型的间充质干细胞标志物，即CD105、CD73、CD90和CD44。尽管hUC-MSCs和hC-MSCs在形态和免疫表型方面相同，但hUC-MSCs的细胞生长速度高于hC-MSCs。通过离子通道的mRNA表达进一步研究了这两种细胞固有的心脏再生潜力。逆转录聚合酶链反应（RT-PCR）结果表明，两种间充质干细胞均表达显著水平的延迟整流样钾离子电流（IKDR）离子通道，但hUC-MSCs和hC-MSCs之间的相对表达水平差异很大，Kv1.1（39±0.6对31±0.8）、Kv2.1（6±0.2对21±0.12）、Kv1.5（7.4±0.1对6.8±0.06）和Kv7.3（27±0.8对13.8±0.6）。同样，两组中也检测到了钙激活钾离子电流（IKCa）通道编码基因、瞬时外向钾离子电流（Ito）和河豚毒素敏感的瞬时内向钠离子电流（INa.TTX）编码基因（Kv4.2、Kv4.3和hNE-Na）的表达。尽管形态和表型相似，但本研究也证实了未分化的hUC-MSCs与hC-MSCs一样存在多种功能性离子通道电流IKDR、IKCa、Ito和INa.TTX。因此，hUC-MSCs可作为未来心脏再生的潜在候选细胞。
