Bauman Cathy A, Jones-Bitton Andria, Menzies Paula, Toft Nils, Jansen Jocelyn, Kelton David
Department of Population Medicine, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1 (Bauman, Jones-Bitton, Menzies, Kelton); Section of Veterinary Epidemiology, Technical University of Denmark, Bülowsvej 27, 1870 Frederiksberg C, Denmark (Toft); Ontario Ministry of Agriculture and Food, 6484 Wellington Road 7, Elora, Ontario N0B 1S0 (Jansen).
Can Vet J. 2016 Feb;57(2):169-75.
A cross-sectional study was undertaken (October 2010 to August 2011) to estimate the prevalence of paratuberculosis in the small ruminant dairy industries in Ontario, Canada. Blood and feces were sampled from 580 goats and 397 sheep (lactating and 2 y of age or older) that were randomly selected from 29 randomly selected dairy goat herds and 21 convenience-selected dairy sheep flocks. Fecal samples were analyzed using bacterial culture (BD BACTEC MGIT 960) and polymerase chain reaction (Tetracore); serum samples were tested with the Prionics Parachek enzyme-linked immunosorbent assay (ELISA). Using 3-test latent class Bayesian models, true farm-level prevalence was estimated to be 83.0% [95% probability interval (PI): 62.6% to 98.1%] for dairy goats and 66.8% (95% PI: 41.6% to 91.4%) for dairy sheep. The within-farm true prevalence for dairy goats was 35.2% (95% PI: 23.0% to 49.8%) and for dairy sheep was 48.3% (95% PI: 27.6% to 74.3%). These data indicate that a paratuberculosis control program for small ruminants is needed in Ontario.
开展了一项横断面研究(2010年10月至2011年8月),以估计加拿大安大略省小型反刍动物奶业中副结核病的患病率。从29个随机选取的奶山羊群和21个便利选取的奶绵羊群中随机抽取了580只山羊和397只绵羊(泌乳期以及2岁及以上),采集血液和粪便样本。粪便样本采用细菌培养法(BD BACTEC MGIT 960)和聚合酶链反应法(Tetracore)进行分析;血清样本采用Prionics Parachek酶联免疫吸附测定(ELISA)进行检测。使用三检验潜在类别贝叶斯模型,估计奶山羊的农场实际患病率为83.0% [95%概率区间(PI):62.6%至98.1%],奶绵羊为66.8%(95% PI:41.6%至91.4%)。奶山羊的农场内实际患病率为35.2%(95% PI:23.0%至49.8%),奶绵羊为48.3%(95% PI:27.6%至74.3%)。这些数据表明,安大略省需要针对小型反刍动物制定副结核病控制计划。