乙型肝炎病毒e抗原在大肠杆菌中的合成。
Synthesis of hepatitis B virus e antigen in E. coli.
作者信息
Inada T, Misumi Y, Seno M, Kanezaki S, Shibata Y, Oka Y, Onda H
机构信息
Nihon Pharmaceutical Company, Research Division, Tokyo, Japan.
出版信息
Virus Res. 1989 Sep;14(1):27-47. doi: 10.1016/0168-1702(89)90067-1.
Hepatitis B virus core antigen (HBcAg) gene was deleted at some unique restriction enzyme sites, or at random, and inserted into the expression plasmids of E. coli which had the tryptophan promoter. E. coli transformants with the plasmids, synthesized materials with many kinds of antigenicity of HBcAg, HBeAg, or both HBcAg and HBeAg. HBeAg-specific material smaller than native HBeAg was produced in a stable condition.
乙肝病毒核心抗原（HBcAg）基因在一些独特的限制性酶切位点被删除，或者随机删除，并插入到具有色氨酸启动子的大肠杆菌表达质粒中。带有这些质粒的大肠杆菌转化体合成了具有多种HBcAg、HBeAg抗原性的物质，或者同时具有HBcAg和HBeAg的抗原性。产生了比天然HBeAg小的、处于稳定状态的HBeAg特异性物质。
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