CD33(+) /HLA-DR(阴性)和CD33(+) /HLA-DR(弱阳性)细胞：人蜕膜中具有髓系来源抑制细胞特征的罕见细胞群。
CD33(+) /HLA-DR(neg) and CD33(+) /HLA-DR(+/-) Cells: Rare Populations in the Human Decidua with Characteristics of MDSC.
作者信息
Bartmann Catharina, Junker Markus, Segerer Sabine E, Häusler Sebastian F, Krockenberger Mathias, Kämmerer Ulrike
机构信息
Department of OB/Gyn, University Hospital of Würzburg, Würzburg, Germany.
MVZ Endokrinologikum Hamburg, Hamburg, Germany.
出版信息
Am J Reprod Immunol. 2016 May;75(5):539-56. doi: 10.1111/aji.12492. Epub 2016 Feb 3.
PROBLEM
Human pregnancy needs a remarkable local immune tolerance toward the conceptus. Myeloid-derived suppressor cells (MDSC) are important players promoting cancer initiation and progression by suppressing T-cell functions and thus inducing immune tolerance. Therefore, MDSC were expected within decidua.
METHODS
Subpopulations of CD33(+) immune cells were isolated from human early pregnancy decidua and characterized phenotypically and functionally by microscopy, FACS analysis, RT-PCR, Western blotting and in the coculture with T cells.
RESULTS
Decidua harbors CD33(+) /HLA-DR(neg) and CD33(+) /HLA-DR(+/-) cells which both express arginase, iNOS and IDO and a typical cytokine profile. Both subtypes potently suppress T-cell proliferation and therefore fulfill the criteria of MDSC.
CONCLUSION
We characterized a new population of CD33(+) /HLA-DR(neg) and CD33(+) /HLA-DR(+/-) cells in human early pregnancy decidua with properties of classical MDSC and thus potentially being an important player in immune tolerance in pregnancy.
问题
人类妊娠需要对胚胎具有显著的局部免疫耐受性。髓系来源的抑制性细胞（MDSC）是通过抑制T细胞功能从而诱导免疫耐受来促进癌症起始和进展的重要参与者。因此，预计蜕膜中存在MDSC。
方法
从人类早孕蜕膜中分离出CD33(+)免疫细胞亚群，并通过显微镜检查、流式细胞术分析、逆转录-聚合酶链反应、蛋白质免疫印迹以及与T细胞共培养对其进行表型和功能鉴定。
结果
蜕膜中含有CD33(+) /HLA-DR(neg)和CD33(+) /HLA-DR(+/-)细胞，这两种细胞均表达精氨酸酶、诱导型一氧化氮合酶和吲哚胺2,3-双加氧酶以及典型的细胞因子谱。这两种亚型均能有效抑制T细胞增殖，因此符合MDSC的标准。
结论
我们鉴定了人类早孕蜕膜中一群新的CD33(+) /HLA-DR(neg)和CD33(+) /HLA-DR(+/-)细胞，它们具有经典MDSC的特性，因此可能是妊娠免疫耐受中的重要参与者。
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