使用适配体-抗体夹心分析法，通过纳米颗粒增强表面等离子体共振检测C反应蛋白。
Detection of C-reactive protein using nanoparticle-enhanced surface plasmon resonance using an aptamer-antibody sandwich assay.
作者信息
Wu Bin, Jiang Rui, Wang Qing, Huang Jin, Yang Xiaohai, Wang Kemin, Li Wenshan, Chen Nandi, Li Qing
机构信息
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha, 410082, China.
出版信息
Chem Commun (Camb). 2016 Feb 28;52(17):3568-71. doi: 10.1039/c5cc10486f. Epub 2016 Feb 4.
High affinity DNA aptamers against C-reactive protein (CRP) were obtained using a microfluidic chip. The aptamers were then used for the construction of an Au nanoparticle enhanced surface plasmon resonance biosensor, which was introduced for the detection of CRP at concentrations ranging from 10 pM to 100 nM in diluted human serum.
利用微流控芯片获得了针对C反应蛋白（CRP）的高亲和力DNA适配体。然后将这些适配体用于构建金纳米颗粒增强表面等离子体共振生物传感器，该传感器用于检测稀释人血清中浓度范围为10 pM至100 nM的CRP。
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