Tatsumi N, Tsuda I, Kojima K, Niri M, Setoguchi K
Med Lab Sci. 1989 Apr;46(2):157-60.
We evaluated the counting of reticulocytes in the peripheral blood with a newly developed flow cytometer type of automated counter that performs a single test within 60 s. The volume of sample needed is 100 microliters and the cells are stained with auramine-O in the counter. The mean within-run reproducibility was 4.66% (CV, n = 50), and dilution of blood gave highly linear results with an r value of 0.996. Correlation was good between manual reticulocyte counts and those performed with the counter (r = 0.893). Samples with large numbers of leucocytes, erythrocytes, or platelets did not interfere with the automated reticulocyte counting, and provided accurate and precise data.
我们使用新开发的流式细胞仪类型的自动计数器评估外周血中网织红细胞的计数，该计数器可在60秒内完成单次检测。所需样本体积为100微升，细胞在计数器中用金胺-O染色。批内重复性均值为4.66%（CV，n = 50），血液稀释呈现高度线性结果，r值为0.996。手工网织红细胞计数与计数器计数之间的相关性良好（r = 0.893）。含有大量白细胞、红细胞或血小板的样本不会干扰自动网织红细胞计数，并能提供准确精确的数据。
