石墨烯量子点作为添加剂用于毛细管电泳分离肉桂酸及其衍生物。

Graphene quantum dots as additives in capillary electrophoresis for separation cinnamic acid and its derivatives.

作者信息

Sun Yaming, Bi Qing, Zhang Xiaoli, Wang Litao, Zhang Xia, Dong Shuqing, Zhao Liang

机构信息

Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, People's Republic of China; University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, People's Republic of China.

Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730000, People's Republic of China.

出版信息

Anal Biochem. 2016 May 1;500:38-44. doi: 10.1016/j.ab.2016.01.024. Epub 2016 Feb 16.

DOI:10.1016/j.ab.2016.01.024

PMID:26893106

Abstract

A facile capillary electrophoresis (CE) method for the separation of cinnamic acid and its derivatives (3,4-dimethoxycinnamic acid, 4-methoxycinnamic acid, isoferulic acid, sinapic acid, cinnamic acid, ferulic acid, and trans-4-hydroxycinnamic acid) using graphene quantum dots (GQDs) as additives with direct ultraviolet (UV) detection is reported. GQDs were synthesized by chemical oxidization and further purified by a macroporous resin column to remove salts (Na2SO4 and NaNO3) and other impurities. Transmission electron microscopy (TEM) indicated that GQDs have a relatively uniform particle size (2.3 nm). Taking into account the structural features of GQDs, cinnamic acid and its derivatives were adopted as model compounds to investigate whether GQDs can be used to improve CE separations. The separation performance of GQDs used as additives in CE was studied through variations of pH, concentration of the background electrolyte (BGE), and contents of GQDs. The results indicated that excellent separation can be achieved in less than 18 min, which is mainly attributed to the interaction between the analytes and GQDs, especially isoferulic acid, sinapic acid, and cinnamic acid.

摘要

报道了一种简便的毛细管电泳（CE）方法，该方法使用石墨烯量子点（GQDs）作为添加剂，通过直接紫外（UV）检测来分离肉桂酸及其衍生物（3,4-二甲氧基肉桂酸、4-甲氧基肉桂酸、异阿魏酸、芥子酸、肉桂酸、阿魏酸和反式-4-羟基肉桂酸）。通过化学氧化合成GQDs，并通过大孔树脂柱进一步纯化以去除盐（Na2SO4和NaNO3）及其他杂质。透射电子显微镜（TEM）表明GQDs具有相对均匀的粒径（2.3 nm）。考虑到GQDs的结构特征，采用肉桂酸及其衍生物作为模型化合物来研究GQDs是否可用于改善CE分离。通过改变pH值、背景电解质（BGE）浓度和GQDs含量，研究了GQDs作为CE添加剂的分离性能。结果表明，在不到18分钟内即可实现出色的分离，这主要归因于分析物与GQDs之间的相互作用，尤其是异阿魏酸、芥子酸和肉桂酸。