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研究从波斯湾采集的四角蛤蜊中与原油共生的降解细菌。

Study the symbiotic crude oil-degrading bacteria in the mussel Mactra stultorum collected from the Persian Gulf.

作者信息

Bayat Zeynab, Hassanshahian Mehdi, Hesni Majid Askari

机构信息

Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.

Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran.

出版信息

Mar Pollut Bull. 2016 Apr 15;105(1):120-4. doi: 10.1016/j.marpolbul.2016.02.042. Epub 2016 Feb 24.

Abstract

Symbiotic associations are complex partnerships that can lead to new metabolic capabilities and the establishment of novel organisms. The diversity of these associations is very broad and there are still many mysteries about the origin and the exact relationship between the organisms that are involved in a symbiosis. The aim of the present study is to find symbiotic crude-oil degrading bacteria in the mussels that collected from the Persian Gulf. Fifteen crude-oil degrading bacteria were isolated from Mactra stultorum mussel that collected from oil contaminated area at Persian Gulf. According to high growth rate on crude oil five strains were selected from 15 isolated strains for more study. Determination of the nucleotide sequence of the gene encoding 16S rRNA show that these isolated strains belong to: Alcanivorax dieselolei strain BHA25, Idiomarina baltica strain BHA28, A. dieselolei strain BHA30, Alcanivorax sp. strain BHA32 and Vibrio azureus strain BHA36. Analysis of remaining of crude oil by Gas Chromatography (GC) confirmed that these strains can degrade: 64%, 63%, 71%, 58% and 75% of crude oil respectively.

摘要

共生关系是复杂的伙伴关系,可带来新的代谢能力并形成新的生物体。这些关系的多样性非常广泛,关于共生中所涉及生物体的起源及确切关系仍存在许多谜团。本研究的目的是在从波斯湾采集的贻贝中寻找共生的原油降解细菌。从采自波斯湾石油污染区域的四角蛤蜊贻贝中分离出了15株原油降解细菌。根据在原油上的高生长速率,从15株分离菌株中挑选出5株进行进一步研究。对编码16S rRNA的基因进行核苷酸序列测定表明,这些分离菌株分别属于:柴油嗜油菌BHA25菌株、波罗的海嗜压菌BHA28菌株、柴油嗜油菌BHA30菌株、嗜油菌属BHA32菌株和天蓝弧菌BHA36菌株。通过气相色谱(GC)对剩余原油进行分析证实,这些菌株分别可降解64%、63%、71%、58%和75%的原油。

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