Parreira J R, Bouraada J, Fitzpatrick M A, Silvestre S, Bernardes da Silva A, Marques da Silva J, Almeida A M, Fevereiro P, Altelaar A F M, Araújo S S
Plant Cell Biotechnology Laboratory, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB-NOVA), Av. da República, Estação Agronómica Nacional, 2780-157 Oeiras, Portugal.
Biomolecular Mass Spectrometry and Proteomics, Bijvoet Centre for Biomolecular Research, Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Netherlands Proteomics Centre, Padualaan 8, 3584CH Utrecht, The Netherlands.
J Proteomics. 2016 Jun 30;143:188-198. doi: 10.1016/j.jprot.2016.03.002. Epub 2016 Mar 3.
Common bean (Phaseolus vulgaris L.) is one of the most consumed staple foods worldwide. Little is known about the molecular mechanisms controlling seed development. This study aims to comprehensively describe proteome dynamics during seed development of common bean. A high-throughput gel-free proteomics approach (LC-MS/MS) was conducted on seeds at 10, 20, 30 and 40days after anthesis, spanning from late embryogenesis until desiccation. Of the 418 differentially accumulated proteins identified, 255 were characterized, most belonging to protein metabolism. An accumulation of proteins belonging to the MapMan functional categories of "protein", "glycolysis", "TCA", "DNA", "RNA", "cell" and "stress" were found at early seed development stages, reflecting an extensive metabolic activity. In the mid stages, accumulation of storage, signaling, starch synthesis and cell wall-related proteins stood out. In the later stages, an increase in proteins related to redox, protein degradation/modification/folding and nucleic acid metabolisms reflect that seed desiccation-resistance mechanisms were activated. Our study unveils new clues to understand the regulation of seed development mediated by post-translational modifications and maintenance of genome integrity. This knowledge enhances the understanding on seed development molecular mechanisms that may be used in the design and selection of common bean seeds with desired quality traits.
Common bean (P. vulgaris) is an important source of proteins and carbohydrates worldwide. Despite the agronomic and economic importance of this pulse, knowledge on common bean seed development is limited. Herein, a gel-free high throughput methodology was used to describe the proteome changes during P. vulgaris seed development. Data obtained will enhance the knowledge on the molecular mechanisms controlling this grain legume seed development and may be used in the design and selection of common bean seeds with desired quality traits. Results may be extrapolated to other pulses.
菜豆（Phaseolus vulgaris L.）是全球消费最多的主食之一。人们对控制种子发育的分子机制知之甚少。本研究旨在全面描述菜豆种子发育过程中的蛋白质组动态变化。采用高通量无胶蛋白质组学方法（LC-MS/MS），对开花后10、20、30和40天的种子进行分析，涵盖从胚胎发育后期到干燥阶段。在鉴定出的418种差异积累蛋白质中，255种得到了表征，大多数属于蛋白质代谢。在种子发育早期，发现属于MapMan功能类别“蛋白质”“糖酵解”“三羧酸循环”“DNA”“RNA”“细胞”和“胁迫”的蛋白质积累，反映出广泛的代谢活动。在中期，储存、信号传导、淀粉合成和细胞壁相关蛋白质的积累较为突出。在后期，与氧化还原、蛋白质降解/修饰/折叠和核酸代谢相关的蛋白质增加，表明种子抗干燥机制被激活。我们的研究揭示了理解翻译后修饰介导的种子发育调控和基因组完整性维持的新线索。这些知识增进了对种子发育分子机制的理解，可用于设计和选择具有所需品质性状的菜豆种子。
菜豆（P. vulgaris）是全球重要的蛋白质和碳水化合物来源。尽管这种豆类在农业和经济上具有重要意义，但关于菜豆种子发育的知识有限。在此，采用无胶高通量方法描述了菜豆种子发育过程中的蛋白质组变化。所获得的数据将增进对控制这种豆科植物种子发育分子机制的了解，并可用于设计和选择具有所需品质性状的菜豆种子。结果可能适用于其他豆类。
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