Xu Zengping, Gao Yuxiang, Meng Shuai, Yang Baochen, Pang Liyun, Wang Chen, Liu Tianjun
Tianjin Key Laboratory of Biomedical Material, Institute of Biomedical Engineering, Peking Union Medical College - Chinese Academy of Medical Sciences Tianjin, China.
Department of Materials Chemistry, Nankai University Tianjin, China.
Front Microbiol. 2016 Mar 2;7:242. doi: 10.3389/fmicb.2016.00242. eCollection 2016.
Lysine-porphyrin conjugate 4i has potent photosensitive antibacterial effect on clinical isolated bacterial strains such as Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, and Pseudomonas aeruginosa. The mechanism of photodynamic antibacterial chemotherapy of 4i (4i-PACT) in vitro and the treatment effect in vivo was investigated in this paper. Atomic force microscopy (AFM) revealed that 4i-PACT can effectively destroy membrane and wall of bacteria, resulting in leakage of its content. This was confirmed by dual fluorescent staining with acridine orange/ethidium bromide and measuring materials absorption at 260 nm. Agarose gel electrophoresis measurement showed that 4i-PACT can damage genomic DNA. Healing of wound in rat infected by mixed bacteria showed that the efficiency of 4i-PACT is dependent on the dose of light. These results showed that 4i-PACT has promising bactericidal effect both in vitro and in vivo.
赖氨酸-卟啉共轭物4i对临床分离的细菌菌株，如耐甲氧西林金黄色葡萄球菌（MRSA）、大肠杆菌和铜绿假单胞菌具有强大的光敏抗菌作用。本文研究了4i光动力抗菌化疗（4i-PACT）的体外作用机制及体内治疗效果。原子力显微镜（AFM）显示，4i-PACT可有效破坏细菌的细胞膜和细胞壁，导致其内容物泄漏。吖啶橙/溴化乙锭双重荧光染色及260nm处物质吸收测量证实了这一点。琼脂糖凝胶电泳测量表明，4i-PACT可损伤基因组DNA。混合细菌感染大鼠伤口的愈合情况表明，4i-PACT的疗效取决于光照剂量。这些结果表明，4i-PACT在体外和体内均具有良好的杀菌效果。
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