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用于发现猪笼草×斑豹猪笼草中植物食虫基因的RNA测序分析。

RNA-seq analysis for plant carnivory gene discovery in Nepenthes × ventrata.

作者信息

Wan Zakaria Wan Nor Adibah, Loke Kok-Keong, Goh Hoe-Han, Mohd Noor Normah

机构信息

Institute of Systems Biology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia.

出版信息

Genom Data. 2015 Nov 10;7:18-9. doi: 10.1016/j.gdata.2015.11.007. eCollection 2016 Mar.

Abstract

Carnivorous plants have the ability to capture and digest insects for nutrients, which allows them to survive in land deprived of nitrogenous nutrients. Nepenthes spp. are one of the carnivorous plants, which uniquely produce pitcher from the tip of an elongated leaf. This study provides the first transcriptome resource from pitcher of a Nepenthes ventricosa × Nepenthes alata hybrid, Nepenthes × ventrata to understand carnivory mechanism in Nepenthes spp., as well as in other carnivorous species. Raw reads and the transcriptome assembly project have been deposited to SRA database with the accession numbers SRX1389337 (day 0 control), SRX1389392 (day 3 longevity), and SRX1389395 (day 3 chitin-treated).

摘要

食虫植物有能力捕捉并消化昆虫以获取养分,这使它们能够在缺乏含氮养分的土地上生存。猪笼草属植物是食虫植物之一,其独特之处在于从细长叶片的尖端长出捕虫笼。本研究提供了首个来自红瓶猪笼草(Nepenthes ventricosa)与菲律宾猪笼草(Nepenthes alata)杂交种——斑豹猪笼草(Nepenthes × ventrata)捕虫笼的转录组资源,以了解猪笼草属植物以及其他食虫物种的食肉机制。原始 reads 和转录组组装项目已存入 SRA 数据库,登录号分别为 SRX1389337(第 0 天对照)、SRX1389392(第 3 天寿命)和 SRX1389395(第 3 天几丁质处理)。

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