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[霉菌毒素的化学分析研究(XX)。玉米及玉米制品中黄曲霉毒素的化学分析]

[Studies on chemical analysis of mycotoxin (XX). Chemical analysis of aflatoxins in corn and corn-products].

作者信息

Isohata E, Toyoda M, Saito Y

出版信息

Eisei Shikenjo Hokoku. 1989(107):128-32.

PMID:2700077
Abstract

The investigation was carried out to analyze aflatoxins contaminated corn. 1) Methanol, chloroform and acetonitrile were used as the extraction solvents for aflatoxins added to corn. As the result, the method using acetonitrile was found to be effective to extract and detect aflatoxins. 2) The Rapid detection of aflatoxins was made possible by using the affinity column (containing monoclonal antibodies against aflatoxins bound onto agarose) and the mini-column. The detection-limit was 20 ppb of aflatoxins in samples.

摘要

开展该调查以分析受黄曲霉毒素污染的玉米。1)甲醇、氯仿和乙腈用作添加到玉米中的黄曲霉毒素的萃取溶剂。结果发现,使用乙腈的方法对黄曲霉毒素的萃取和检测有效。2)通过使用亲和柱(含有结合在琼脂糖上的抗黄曲霉毒素单克隆抗体)和微型柱能够快速检测黄曲霉毒素。样品中黄曲霉毒素的检测限为20 ppb。

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