Fu Qinyi, Martin Benjamin L, Matus David Q, Gao Liang
Department of Chemistry, Stony Brook University, Stony Brook, New York 11794, USA.
Department of Biochemistry &Cell Biology, Stony Brook University, Stony Brook, New York 11794, USA.
Nat Commun. 2016 Mar 23;7:11088. doi: 10.1038/ncomms11088.
Despite the progress made in selective plane illumination microscopy, high-resolution 3D live imaging of multicellular specimens remains challenging. Tiling light-sheet selective plane illumination microscopy (TLS-SPIM) with real-time light-sheet optimization was developed to respond to the challenge. It improves the 3D imaging ability of SPIM in resolving complex structures and optimizes SPIM live imaging performance by using a real-time adjustable tiling light sheet and creating a flexible compromise between spatial and temporal resolution. We demonstrate the 3D live imaging ability of TLS-SPIM by imaging cellular and subcellular behaviours in live C. elegans and zebrafish embryos, and show how TLS-SPIM can facilitate cell biology research in multicellular specimens by studying left-right symmetry breaking behaviour of C. elegans embryos.
尽管在选择性平面照明显微镜方面取得了进展，但对多细胞样本进行高分辨率三维实时成像仍然具有挑战性。为应对这一挑战，人们开发了具有实时光片优化功能的平铺光片选择性平面照明显微镜（TLS-SPIM）。它通过使用实时可调的平铺光片，在空间分辨率和时间分辨率之间实现灵活折衷，从而提高了选择性平面照明显微镜在解析复杂结构方面的三维成像能力，并优化了选择性平面照明显微镜的实时成像性能。我们通过对活的秀丽隐杆线虫和斑马鱼胚胎中的细胞及亚细胞行为进行成像，展示了TLS-SPIM的三维实时成像能力，并通过研究秀丽隐杆线虫胚胎的左右对称破缺行为，展示了TLS-SPIM如何促进多细胞样本中的细胞生物学研究。
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