用于靶向药物递送的肺癌特异性景观噬菌体的筛选。
Selection of Lung Cancer-Specific Landscape Phage for Targeted Drug Delivery.
作者信息
Gillespie James W, Wei Lixia, Petrenko Valery A
机构信息
Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA.
出版信息
Comb Chem High Throughput Screen. 2016;19(5):412-22. doi: 10.2174/1386207319666160420141024.
Cancer cell-specific diagnostic or therapeutic tools are commonly believed to significantly increase the success rate of cancer diagnosis and targeted therapies. To extend the repertoire of available cancer cell-specific phage fusion proteins and study their efficacy as navigating moieties, we used two landscape phage display libraries f8/8 and f8/9 displaying an 8- or 9-mer random peptide fusion to identify a panel of novel peptide families that are specific to Calu-3 cells. Using a phage capture assay, we showed that two of the selected phage clones, ANGRPSMT and VNGRAEAP (phage and their recombinant proteins are named by the sequence of the fusion peptide), are selective for the Calu-3 cell line in comparison to phenotypically normal lung epithelial cells and distribute into unique subcellular fractions.
癌细胞特异性诊断或治疗工具通常被认为能显著提高癌症诊断和靶向治疗的成功率。为了扩展可用的癌细胞特异性噬菌体融合蛋白库，并研究它们作为导航部分的功效，我们使用了两个展示8肽或9肽随机肽融合的全景噬菌体展示文库f8/8和f8/9，以鉴定一组对Calu-3细胞特异的新型肽家族。通过噬菌体捕获试验，我们发现，与表型正常的肺上皮细胞相比，所选的两个噬菌体克隆ANGRPSMT和VNGRAEAP（噬菌体及其重组蛋白由融合肽序列命名）对Calu-3细胞系具有选择性，并分布到独特的亚细胞组分中。
Zotero 插件