Walkiewicz Katarzyna, Kozieł Paweł, Bednarczyk Martyna, Błażelonis Adam, Mazurek Urszula, Muc-Wierzgoń Małgorzata
Department of Internal Medicine, Faculty of Public Health in Bytom, Medical University of Silesia, 40-055 Katowice, Poland.
Department of Molecular Biology, Faculty of Pharmacy in Sosnowiec, Medical University of Silesia, 40-055 Katowice, Poland.
Biomed Res Int. 2016;2016:8208904. doi: 10.1155/2016/8208904. Epub 2016 Mar 27.
The ability to form metastases which depends on the mechanisms of cell migration is an important element of the progression of cancer. In the present study we analyzed the genes involved in the regulation of migration in colon cancer cells.
A total of 20 pairs of surgically removed tumoral and healthy (marginal) tissues samples from colorectal cancer patients at clinical stages I-II and III-IV were analyzed. The isolation of RNA from CRC and normal tissues and its subsequent molecular analysis were performed according to manufacturer's instructions. Microarray data analysis was performed using the GeneSpring 11.5 platform and Significance Analysis of Microarrays (SAM). In SAM analysis to identify significantly differentially expressed genes score and q-value parameters were used.
The largest increase in expression of genes was shown by MMP9, ADAM17, EphA2, and TIMP.
Presented genes, especially ADAM17, MMP9, EphA2, TIMP1, ICAM 11, and CD4, may be used as prognostic markers of advanced stages of colorectal cancer, contributing to the development of new lines of therapy focused on reducing metastasis of the primary tumor.
形成转移灶的能力依赖于细胞迁移机制，是癌症进展的一个重要因素。在本研究中，我们分析了参与结肠癌细胞迁移调控的基因。
分析了总共20对从临床I-II期和III-IV期结直肠癌患者手术切除的肿瘤组织和健康（边缘）组织样本。根据制造商说明，从结直肠癌组织和正常组织中分离RNA并进行后续分子分析。使用GeneSpring 11.5平台和微阵列显著性分析（SAM）进行微阵列数据分析。在SAM分析中，使用得分和q值参数来识别显著差异表达的基因。
MMP9、ADAM17、EphA2和TIMP基因的表达增加最为显著。
所呈现的这些基因，尤其是ADAM17、MMP9、EphA2、TIMP1、ICAM 11和CD4，可作为结直肠癌晚期的预后标志物，有助于开发专注于减少原发性肿瘤转移的新治疗方法。
