Nalbant Ayten, Saygılı Tahsin
Molecular Immunology and Gene Regulation Laboratory, Department of Molecular Biology and Genetics, Izmir Institute of Technology, Urla, İzmir, 35430 Turkey.
Alexion Ilac Ticaret Ltd Sti., Buyukdere Cad. No: 100-102 B Blok Kat:1 Istanbul, 34394 Turkey.
PLoS One. 2016 Apr 27;11(4):e0154085. doi: 10.1371/journal.pone.0154085. eCollection 2016.
Actinobacillus (Aggregatibacter) actinomycetemicomitans (Aa) is a bacterium that lives in the oral cavity and plays an important role in periodontal diseases. The effect of A.actinomycetemcomitans's heat shock family protein GroEL on host or immune cells including monocytes is quite limited. In this study, the recombinant A.actinomycetemcomitans's GroEL protein (rAaGroEL) was used as an antigen and its effects on monocytes of peripheral blood mononuclear cells (PBMCs) was investigated. To do that, PBMCs were stimulated with rAaGroEL protein at different time points (16h to 96h) and the cytokines of CD14+ monocytes were detected with intracellular cytokine staining by Flow cytometry. Data showed that AaGroEL protein has an antigenic effect on human primary monocytes. AaGroEL protein responsive CD14 monocytes stimulates the expression of IL12, IL10, IFNγ and TNFα cytokines with different kinetics and expression profile. Therefore, A. actinomycetemcomitans's heat shock GroEL protein can modulate innate and adaptive immune responses and contribute to an inflammatory diseases pathology.
伴放线放线杆菌（聚集杆菌属）伴放线放线杆菌（Aa）是一种存在于口腔中且在牙周疾病中起重要作用的细菌。伴放线放线杆菌的热休克家族蛋白GroEL对包括单核细胞在内的宿主或免疫细胞的影响相当有限。在本研究中，重组伴放线放线杆菌的GroEL蛋白（rAaGroEL）被用作抗原，并研究了其对外周血单核细胞（PBMC）中单核细胞的影响。为此，在不同时间点（16小时至96小时）用rAaGroEL蛋白刺激PBMC，并通过流式细胞术的细胞内细胞因子染色检测CD14 +单核细胞的细胞因子。数据表明，AaGroEL蛋白对人原代单核细胞具有抗原作用。对AaGroEL蛋白有反应的CD14单核细胞以不同的动力学和表达谱刺激IL12、IL10、IFNγ和TNFα细胞因子的表达。因此，伴放线放线杆菌的热休克GroEL蛋白可调节先天性和适应性免疫反应，并导致炎症性疾病病理过程。
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