Liang Ying, Su Jing, Huang Yong, Li Xiaohua, Tao Yiwen, Lu Chaofeng, Zhu Jianmeng, Bai Zhihao, Meng Jinyu, Lu Xiaoling, Zhao Yongxiang
National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University.
Anal Sci. 2016;32(5):565-9. doi: 10.2116/analsci.32.565.
In the present work, a sensitive electrochemical aptasensor was designed for the detection of adenosine triphosphate (ATP) with hemin/graphene oxide nanosheets (HGNs). Firstly, the ATP aptamer was self-assembled on gold electrode surface, and then HGNs were captured to the modified electrode by π-π stacking. The captured HGNs could catalyze the disproportionation reaction of H2O2, and produced a detectable electrochemical signal by chronoamperometry. ATP was competitively bound to aptamer which led to the release of HGNs from the electrode surface after adding ATP. The decrease of the electrochemical signal, which was calculated by the difference of amperometric responses before and after incubation of ATP, provided a quantitative signal for ATP detection. A linear correlation was achieved between the difference of the amperometric responses and the logarithmic concentration of ATP ranging from 0.5 to 100 nM with a detection limit of 0.08 nM. Besides, the aptasensor also exhibited good selectivity toward ATP against other analogs.
在本工作中，设计了一种灵敏的电化学适体传感器，用于使用血红素/氧化石墨烯纳米片（HGNs）检测三磷酸腺苷（ATP）。首先，ATP适配体自组装在金电极表面，然后通过π-π堆积将HGNs捕获到修饰电极上。捕获的HGNs可以催化H2O2的歧化反应，并通过计时电流法产生可检测的电化学信号。加入ATP后，ATP与适配体竞争性结合，导致HGNs从电极表面释放。通过ATP孵育前后安培响应的差异计算得到的电化学信号的降低，为ATP检测提供了定量信号。安培响应的差异与ATP对数浓度在0.5至100 nM范围内呈线性相关，检测限为0.08 nM。此外，该适体传感器对ATP相对于其他类似物也表现出良好的选择性。
