Kołodziejczyk Lidia, Podraza Wojciech, Gonet Bolesław, Dzika Ewa, Kosik-Bogacka Danuta I
Folia Biol (Krakow). 2016;64(1):55-8. doi: 10.3409/fb64_1.55.
The aim of this study was to determine the effect of an extremely low-frequency magnetic field (ELFMF) on the production of liver fluke larvae in a parasite-host system: Fasciola hepatica--Galba truncatula. Both F. hepatica eggs and F. hepatica-infected snails were exposed to an ELFMF (50 Hz, 2.0 mT) for 14 days and 36 days, respectively. F. hepatica-infected snails were divided into 4 groups, 10 specimens each. The snails of groups I and II were infected with F. hepatica larvae--miracidia obtained from control cultures, while the snails of groups III and IV were infected with miracidia reared from eggs that had been incubated in an ELFMF. After infection, the snails of groups II and IV were placed in an ELFMF, while those of groups I (control) and III were housed outside the ELFMF. At 36 days post-infection (dpi) there were no statistically significant differences between the number of F. hepatica larvae--cercariae and metacercariae, obtained from G. truncatula snails in the control group (group I) and the snail groups exposed to ELFMF (groups II, III and IV). However, a statistically significant difference between the average number of F. hepatica larvae in snail groups III and IV may indicate that the duration of exposure to ELFMF, i.e. embryogenesis period vs. the entire larval development, played a role in the production of F. hepatica larvae, and resulted in a reduction of their number.
本研究的目的是确定极低频磁场(ELFMF)对寄生虫-宿主系统中肝片吸虫幼虫产生的影响:肝片吸虫——截口圆扁螺。分别将肝片吸虫卵和感染肝片吸虫的蜗牛暴露于ELFMF(50 Hz,2.0 mT)中14天和36天。将感染肝片吸虫的蜗牛分为4组,每组10只。第I组和第II组的蜗牛感染从对照培养物中获得的肝片吸虫幼虫——毛蚴,而第III组和第IV组的蜗牛感染从在ELFMF中孵化的卵中培育出的毛蚴。感染后,第II组和第IV组的蜗牛置于ELFMF中,而第I组(对照组)和第III组的蜗牛饲养在ELFMF外。在感染后36天(dpi),对照组(第I组)的截口圆扁螺蜗牛以及暴露于ELFMF的蜗牛组(第II、III和IV组)产生的肝片吸虫幼虫——尾蚴和后尾蚴数量之间无统计学显著差异。然而,第III组和第IV组蜗牛中肝片吸虫幼虫平均数量之间的统计学显著差异可能表明,暴露于ELFMF的持续时间,即胚胎发育期与整个幼虫发育期,对肝片吸虫幼虫的产生有影响,并导致其数量减少。