Chemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; National Research Centre, Textile Research Division, Pre-treatment and Finishing of Cellulosic Fibers, Dokki, P.O. 12311, Cairo, Egypt.
CEITEC-Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, 616 00 Brno, Czech Republic; SCITEG, a.s., Brno, Czech Republic; National Research Centre, Textile Research Division, Pre-treatment and Finishing of Cellulosic Fibers, Dokki, P.O. 12311, Cairo, Egypt.
Int J Biol Macromol. 2016 Aug;89:582-91. doi: 10.1016/j.ijbiomac.2016.05.021. Epub 2016 May 10.
Recruitment of cells and mediators is altered during impaired wound healing, thereby delaying this process. To overcome this problem, the correlation of wound healing in older rats, and the impact of different molecular weight of hyaluronan without silver nanoparticles; (low-HA1), (High-HA2), (Medium- HA3) and with silver nanoparticles (High-HA4) is investigated. The superior HA were selected to be further investigated onto diabetic wounds. Our results pointed to a marked deficiency in wounds granulation in older rats, which was accompanied with impairment of healing process. In older rats group treated with HA2 or HA4, granulation and dermal construction were improved. Furthermore, the number of pathogenic bacteria on wounds was declined throughout the first 24h by HA2 and HA4. The wound size in HA4-treated older rats was significantly smaller than that in other HA1, HA2 or HA3-treated older ones. Also, diabetes impaired the level of inflammatory cytokine, in diabetic model. On contrary, HA4 was found to normalize the level of inflammatory cytokine, in the diabetic model. Furthermore, HA4 was found to recover all oxidative and toxicity markers in diabetic models. This data confirms the critical role of HA4 to improve granulation and inflammatory mediators in impaired older and diabetic rat wound healing.
在伤口愈合受损的情况下，细胞和介质的募集会发生改变，从而延迟这一过程。为了克服这个问题，我们研究了老年大鼠的伤口愈合相关性，以及不同分子量的透明质酸（无银纳米粒子的低 HA1、高 HA2、中 HA3 和含银纳米粒子的高 HA4）对其的影响。选择性能优越的透明质酸进一步研究其对糖尿病伤口的影响。我们的结果表明，老年大鼠的伤口肉芽形成明显不足，愈合过程受损。在接受 HA2 或 HA4 治疗的老年大鼠组中，肉芽组织和真皮结构得到改善。此外，HA2 和 HA4 在最初 24 小时内可减少伤口处的致病菌数量。与其他 HA1、HA2 或 HA3 治疗的老年大鼠相比，HA4 治疗的老年大鼠的伤口面积明显更小。此外，糖尿病会降低糖尿病模型中的炎症细胞因子水平，而 HA4 则被发现可使炎症细胞因子水平正常化。此外，HA4 还可恢复糖尿病模型中的所有氧化和毒性标志物。这些数据证实了 HA4 在改善老年和糖尿病大鼠伤口愈合中受损的肉芽组织和炎症介质方面的关键作用。
