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白鲑(Coregonus maraena)的转录组测序

Transcriptome sequencing of maraena whitefish (Coregonus maraena).

作者信息

Brietzke Andreas, Borchel Andreas, Altmann Simone, Nipkow Mareen, Rebl Alexander, Brunner Ronald M, Goldammer Tom

机构信息

Leibniz Institute for Farm Animal Biology, Institute for Genome Biology, Fish Genetics Unit, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.

Leibniz Institute for Farm Animal Biology, Institute for Genome Biology, Fish Genetics Unit, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany; SLRC - Sea Lice Research Centre, Department of Biology, University of Bergen, Thormøhlensgata 55, 5008 Bergen, Norway.

出版信息

Mar Genomics. 2016 Oct;29:27-29. doi: 10.1016/j.margen.2016.05.006. Epub 2016 Jun 4.

Abstract

Maraena whitefish (Coregonus maraena, Bloch, 1779) is a high-quality food fish belonging to the family Salmonidae with considerable economic relevance in the Baltic area. Aquaculture of this species is fundamental for its successful conservation and thus sustainable fisheries. Robust fishes obtained from breeding lines build the basis for effective aquaculture. Doubtless, the utilization of transcriptome sequencing and identification of genetic markers contribute to this aim. 454 FLX Titanium Sequencing provided 1.31 million sequence reads representing a first insight into the C. maraena transcriptome. The 454 Newbler Assembly arranged 29,094 contigs with an average length of 798bp. We found a whole series of transcripts highly probably resulting from ancient genome duplication and annotated 2887 different transcripts with an average length of 812bp. Functional annotation obtained a transcript composition predominantly comprising enzyme-coding genes.

摘要

马雷纳白鲑(Coregonus maraena,布洛赫,1779年)是一种优质食用鱼,属于鲑科,在波罗的海地区具有相当重要的经济意义。该物种的水产养殖对其成功保护以及可持续渔业至关重要。从育种系获得的健壮鱼类是有效水产养殖的基础。毫无疑问,转录组测序的利用和遗传标记的鉴定有助于实现这一目标。454 FLX Titanium测序提供了131万个序列读数,首次深入了解了马雷纳白鲑的转录组。454 Newbler组装软件排列出29094个重叠群,平均长度为798bp。我们发现了一系列很可能源于古代基因组复制的转录本,并注释了2887个不同的转录本,平均长度为812bp。功能注释获得的转录本组成主要包含酶编码基因。

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