Fas和Fas配体基因多态性与子痫前期的关系。

Relationship between Fas and Fas Ligand gene polymorphisms and pre-eclampsia.

作者信息

Masoumi Elham, Tavakkol-Afshari Jalil, Nikpoor Amin Reza, Ghaffari-Nazari Haniyeh, Tahaghoghi-Hajghorbani Sahar, Jalali Seyed Amir

机构信息

Immunogenetic and Cell Culture Department, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Department of Immunology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

J Obstet Gynaecol Res. 2016 Oct;42(10):1272-1278. doi: 10.1111/jog.13062. Epub 2016 Jun 9.

DOI:10.1111/jog.13062

PMID:27277758

Abstract

AIM

In normal pregnancy, the Th1 subtype, responsible for the production of inflammatory cytokines, is reduced, and the Th2 subtype is increased to prohibit inflammation. In pre-eclampsia, the Th1 cell population is increased; thus, subsequent inflammation and trophoblast destruction occur. Polymorphisms in the Fas and Fas Ligand (FasL) promoter regions can influence Fas and FasL expression and accused to increase of Th1 subtype.

METHODS

DNA samples from 153 pregnant women with pre-eclampsia and 140 controls were genotyped through polymerase chain reaction-restriction fragment length polymorphism. A Fisher's exact test was used to compare the distribution of individual polymorphisms.

RESULTS

Fas-1377 AA, AG and GG genotypes were observed in 2.61%, 18.30% and 79.08% in the pre-eclampsia group opposed to 0%, 27.14% and 72.85% in the control group (P = 0.037), respectively. Fas-670 AA, AG and GG genotypes were observed in 37.9%, 41.8% and 20.3% of pre-eclampsia patients compared with 33.6%, 50.7% and 15.7% in healthy pregnant women (P = 0.291), respectively. No statically significant differences in the FasL-844 genotype were observed between the groups (P = 0.69).

CONCLUSION

The Fas-1377G > A polymorphism is associated with a higher risk of pre-eclampsia.

摘要

目的

在正常妊娠中，负责产生炎性细胞因子的Th1亚型减少，而Th2亚型增加以抑制炎症。在子痫前期，Th1细胞群增加；因此，随后会发生炎症和滋养层破坏。Fas和Fas配体（FasL）启动子区域的多态性可影响Fas和FasL的表达，并被认为与Th1亚型的增加有关。

方法

通过聚合酶链反应-限制性片段长度多态性对153例子痫前期孕妇和140例对照组孕妇的DNA样本进行基因分型。采用Fisher精确检验比较个体多态性的分布。

结果

子痫前期组中Fas - 1377 AA、AG和GG基因型的比例分别为2.61%、18.30%和79.08%，而对照组分别为0%、27.14%和72.85%（P = 0.037）。子痫前期患者中Fas - 670 AA、AG和GG基因型的比例分别为37.9%、41.8%和20.3%，健康孕妇中分别为33.6%、50.7%和15.7%（P = 0.291）。两组间FasL - 844基因型无统计学显著差异（P = 0.69）。