巨细胞病毒（CMV）酶联免疫斑点试验而非CMV全血γ干扰素释放试验是确定孕妇先天性CMV感染风险的新型生物标志物。
Cytomegalovirus (CMV) Enzyme-Linked Immunosorbent Spot Assay but Not CMV QuantiFERON Assay Is a Novel Biomarker To Determine Risk of Congenital CMV Infection in Pregnant Women.
作者信息
Forner Gabriella, Saldan Alda, Mengoli Carlo, Gussetti Nadia, Palù Giorgio, Abate Davide
机构信息
Department of Molecular Medicine, University of Padua, Padua, Italy.
Padua Reference Center for Infections in Pregnancy, Padua General Hospital, Padua, Italy.
出版信息
J Clin Microbiol. 2016 Aug;54(8):2149-54. doi: 10.1128/JCM.00561-16. Epub 2016 Jun 8.
Abstract
Cytomegalovirus (CMV) enzyme-linked immunosorbent spot (ELISPOT) and CMV QuantiFERON assays were examined as potential biomarkers predictive of congenital CMV (cCMV) transmission. Fifty-seven pregnant women with primary CMV infection and 23 with nonprimary CMV infection were recruited in the study. Maternal age, CMV IgG avidity, viremia, and viruria were also included among the potential predictors. Spearman's statistical correlation analysis revealed a positive correlation between the CMV ELISPOT and CMV QuantiFERON assay results (P < 0.001), but only the CMV ELISPOT assay correlated with cCMV (P < 0.001). cCMV was positively correlated with maternal viremia and viruria (P < 0.05) and negatively correlated with CMV IgG avidity (P < 0.01). Maternal age and CMV QuantiFERON assay results were not statistically associated with cCMV. CMV-specific cell-mediated immunity detected by the CMV ELISPOT assay plays a critical role in cCMV.
摘要
巨细胞病毒（CMV）酶联免疫斑点试验（ELISPOT）和CMV全血γ干扰素释放试验被作为预测先天性巨细胞病毒（cCMV）传播的潜在生物标志物进行检测。该研究招募了57名原发性CMV感染的孕妇和23名非原发性CMV感染的孕妇。潜在预测因素还包括产妇年龄、CMV IgG亲和力、病毒血症和病毒尿症。Spearman统计相关性分析显示，CMV ELISPOT试验和CMV全血γ干扰素释放试验结果之间呈正相关（P < 0.001），但只有CMV ELISPOT试验与cCMV相关（P < 0.001）。cCMV与产妇病毒血症和病毒尿症呈正相关（P < 0.05），与CMV IgG亲和力呈负相关（P < 0.01）。产妇年龄和CMV全血γ干扰素释放试验结果与cCMV无统计学关联。通过CMV ELISPOT试验检测到的CMV特异性细胞介导免疫在cCMV中起关键作用。
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