Wnt信号通路基因wls、wnt9a、wnt5b、frzb和gpc4在斑马鱼腭部形态发生过程中调节汇聚延伸的作用。
Roles of Wnt pathway genes wls, wnt9a, wnt5b, frzb and gpc4 in regulating convergent-extension during zebrafish palate morphogenesis.
作者信息
Rochard Lucie, Monica Stefanie D, Ling Irving T C, Kong Yawei, Roberson Sara, Harland Richard, Halpern Marnie, Liao Eric C
机构信息
Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Harvard Stem Cell Institute, Boston, MA 02114, USA.
Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720, USA.
出版信息
Development. 2016 Jul 15;143(14):2541-7. doi: 10.1242/dev.137000. Epub 2016 Jun 10.
The Wnt signaling pathway is crucial for tissue morphogenesis, participating in cellular behavior changes, notably during the process of convergent-extension. Interactions between Wnt-secreting and receiving cells during convergent-extension remain elusive. We investigated the role and genetic interactions of Wnt ligands and their trafficking factors Wls, Gpc4 and Frzb in the context of palate morphogenesis in zebrafish. We describe that the chaperon Wls and its ligands Wnt9a and Wnt5b are expressed in the ectoderm, whereas juxtaposed chondrocytes express Frzb and Gpc4. Using wls, gpc4, frzb, wnt9a and wnt5b mutants, we genetically dissected the Wnt signals operating between secreting ectoderm and receiving chondrocytes. Our analysis delineates that non-canonical Wnt signaling is required for cell intercalation, and that wnt5b and wnt9a are required for palate extension in the anteroposterior and transverse axes, respectively.
Wnt信号通路对于组织形态发生至关重要，参与细胞行为变化，尤其是在汇聚延伸过程中。在汇聚延伸过程中，分泌Wnt的细胞与接收Wnt的细胞之间的相互作用仍不清楚。我们研究了Wnt配体及其转运因子Wls、Gpc4和Frzb在斑马鱼腭部形态发生过程中的作用和遗传相互作用。我们描述了伴侣蛋白Wls及其配体Wnt9a和Wnt5b在外胚层中表达，而相邻的软骨细胞表达Frzb和Gpc4。利用wls、gpc4、frzb、wnt9a和wnt5b突变体，我们对分泌外胚层和接收软骨细胞之间起作用的Wnt信号进行了遗传学剖析。我们的分析表明，非经典Wnt信号传导是细胞插入所必需的，并且wnt5b和wnt9a分别是腭部在前后轴和横轴上延伸所必需的。
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