• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

基于中性载体的细胞内镁离子选择性微电极。

Intracellular magnesium ion selective microelectrode based on a neutral carrier.

作者信息

Hu Z M, Bührer T, Müller M, Rusterholz B, Rouilly M, Simon W

出版信息

Anal Chem. 1989 Mar 15;61(6):574-6. doi: 10.1021/ac00181a015.

DOI:10.1021/ac00181a015
PMID:2729592
Abstract

A magnesium ion selective microelectrode based on a synthetic neutral carrier is presented. The selectivity of Mg2+ over Na+, K+, H+, and Ca2+ is sufficient for assays of intracellular magnesium ion activities. The microelectrodes with an optimized membrane composition have a resistance of about 5 x 10(10) omega and a 90% response time of less than or equal to 3 s for a tip diameter around 1 microns. The lifetime of the microelectrode cell assembly is longer than 1 week and the emf drift after equilibration is less than or equal to 0.3 mV/h.

摘要

介绍了一种基于合成中性载体的镁离子选择性微电极。Mg2+ 对 Na+、K+、H+ 和 Ca2+ 的选择性足以用于细胞内镁离子活性的测定。具有优化膜组成的微电极电阻约为 5×10(10) 欧姆,对于尖端直径约为 1 微米的情况,90% 的响应时间小于或等于 3 秒。微电极电池组件的寿命超过 1 周,平衡后的电动势漂移小于或等于 0.3 mV/h。

相似文献

1
Intracellular magnesium ion selective microelectrode based on a neutral carrier.基于中性载体的细胞内镁离子选择性微电极。
Anal Chem. 1989 Mar 15;61(6):574-6. doi: 10.1021/ac00181a015.
2
Neutral carrier sodium ion-selective microelectrode for extracellular studies.
Neurosci Lett. 1985 Jun 24;57(3):267-71. doi: 10.1016/0304-3940(85)90503-8.
3
Neutral carrier Na+- and Ca2+-selective microelectrodes for intracellular application.用于细胞内应用的中性载体钠和钙选择性微电极。
Biophys J. 1982 Dec;40(3):199-207. doi: 10.1016/S0006-3495(82)84475-5.
4
Intracellular neutral carrier-based Ca2+ microelectrode with subnanomolar detection limit.具有亚纳摩尔检测限的基于细胞内中性载体的钙离子微电极。
Pflugers Arch. 1987 Jul;409(3):223-8. doi: 10.1007/BF00583469.
5
Novel magnesium ion-selective microelectrodes based on a neutral carrier.
Pflugers Arch. 1993 May;423(3-4):338-42. doi: 10.1007/BF00374414.
6
Direct measurement of intracellular free magnesium in frog skeletal muscle using magnesium-selective microelectrodes.使用镁选择性微电极直接测量青蛙骨骼肌细胞内的游离镁。
Biochim Biophys Acta. 1984 May 22;804(1):1-7. doi: 10.1016/0167-4889(84)90091-0.
7
Extra- and intracellular hydrogen ion-selective microelectrode based on neutral carriers with extended pH response range in acid media.基于中性载体的细胞外和细胞内氢离子选择性微电极,在酸性介质中具有扩展的pH响应范围。
Pflugers Arch. 1988 Feb;411(2):216-9. doi: 10.1007/BF00582318.
8
Characterization procedure for ion-selective electrode assays of magnesium activity in aqueous solutions of physiological composition.生理成分水溶液中镁活性的离子选择性电极测定法的表征程序。
Clin Chem. 1993 May;39(5):855-9.
9
Estimation of intracellular free magnesium using ion-selective microelectrodes: evidence for an Na/Mg exchange mechanism in skeletal muscle.使用离子选择性微电极评估细胞内游离镁:骨骼肌中钠/镁交换机制的证据
Magnes Trace Elem. 1991;10(2-4):67-79.
10
Intracellular free magnesium in frog skeletal muscle fibres measured with ion-selective micro-electrodes.用离子选择性微电极测量青蛙骨骼肌纤维中的细胞内游离镁。
J Physiol. 1986 Sep;378:461-83. doi: 10.1113/jphysiol.1986.sp016230.

引用本文的文献

1
Electrode chemistry yields a nanoparticle-based NMR sensor for calcium.电极化学产生了一种基于纳米颗粒的钙核磁共振传感器。
Langmuir. 2008 Jul 15;24(14):7596-8. doi: 10.1021/la8006298. Epub 2008 Jun 18.
2
Evidence of a water layer in solid-contact polymeric ion sensors.固体接触聚合物离子传感器中存在水层的证据。
Phys Chem Chem Phys. 2008 Jan 7;10(1):73-6. doi: 10.1039/b714248j. Epub 2007 Oct 19.
3
Novel magnesium ion-selective microelectrodes based on a neutral carrier.
Pflugers Arch. 1993 May;423(3-4):338-42. doi: 10.1007/BF00374414.
4
A new double-barrelled, ionophore-based microelectrode for chloride ions.一种新型的基于离子载体的双管氯离子微电极。
Pflugers Arch. 1989 Sep;414(6):663-8. doi: 10.1007/BF00582133.
5
Intracellular free magnesium in frog skeletal muscle studied with a new type of magnesium-selective microelectrode: interactions between magnesium and sodium in the regulation of [Mg]i.用新型镁选择性微电极研究青蛙骨骼肌细胞内游离镁:镁与钠在调节细胞内镁浓度中的相互作用
Pflugers Arch. 1990 May;416(3):238-46. doi: 10.1007/BF00392059.
6
Intracellular free Mg2+ concentration in skeletal muscle fibres of frog and crayfish.青蛙和小龙虾骨骼肌纤维中的细胞内游离镁离子浓度。
Pflugers Arch. 1991 Jan;417(5):446-53. doi: 10.1007/BF00370938.
7
Myoplasmic free Mg2+ concentration during repetitive stimulation of single fibres from mouse skeletal muscle.小鼠骨骼肌单纤维重复刺激期间肌质游离镁离子浓度
J Physiol. 1992;453:413-34. doi: 10.1113/jphysiol.1992.sp019236.