Salvati Louis M, McClure Sean C, Reddy Todime M, Cellar Nicholas A
Abbott Nutrition, 3300 Stelzer Rd, Columbus, OH 43219, USA.
J AOAC Int. 2016 May;99(3):776-85. doi: 10.5740/jaoacint.15-0315.
This method provides simultaneous determination of total vitamins B1, B2, B3, and B6 in infant formula and related nutritionals (adult and infant). The method was given First Action for vitamins B1, B2, and B6, but not B3, during the AOAC Annual Meeting in September 2015. The method uses acid phosphatase to dephosphorylate the phosphorylated vitamin forms. It then measures thiamine (vitamin B1); riboflavin (vitamin B2); nicotinamide and nicotinic acid (vitamin B3); and pyridoxine, pyridoxal, and pyridoxamine (vitamin B6) from digested sample extract by liquid chromatography-tandem mass spectrometry. A single-laboratory validation was performed on 14 matrixes provided by the AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) to demonstrate method effectiveness. The method met requirements of the AOAC SPIFAN Standard Method Performance Requirement for each of the three vitamins, including average over-spike recovery of 99.6 ± 3.5%, average repeatability of 1.5 ± 0.8% relative standard deviation, and average intermediate precision of 3.9 ± 1.3% relative standard deviation.
该方法可同时测定婴儿配方奶粉及相关营养制品（成人和婴儿）中的维生素B1、B2、B3和B6总量。在2015年9月的AOAC年会上，该方法被首次批准用于维生素B1、B2和B6的测定，但未批准用于维生素B3。该方法使用酸性磷酸酶使磷酸化的维生素形式去磷酸化。然后通过液相色谱-串联质谱法测定消化后的样品提取物中的硫胺素（维生素B1）、核黄素（维生素B2）、烟酰胺和烟酸（维生素B3）以及吡哆醇、吡哆醛和吡哆胺（维生素B6）。对AOAC婴儿配方奶粉和成人营养制品利益相关方小组（SPIFAN）提供的14种基质进行了单实验室验证，以证明该方法的有效性。该方法满足了AOAC SPIFAN对三种维生素中每种维生素的标准方法性能要求，包括平均加标回收率为99.6±3.5%、平均重复性相对标准偏差为1.5±0.8%以及平均中间精密度相对标准偏差为3.9±1.3%。
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