壳寡糖可提高结肠癌SW480细胞系的放射敏感性。
Chitooligosaccharides promote radiosensitivity in colon cancer line SW480.
作者信息
Han Fu-Shi, Yang Shi-Jie, Lin Mou-Bin, Chen Ying-Qun, Yang Ping, Xu Jin-Ming
机构信息
Fu-Shi Han, Jin-Ming Xu, Department of Imaging, Tongji University Affiliated Yangpu Hospital, Shanghai 200090, China.
出版信息
World J Gastroenterol. 2016 Jun 14;22(22):5193-200. doi: 10.3748/wjg.v22.i22.5193.
AIM
To investigate the anti-proliferation and radiosensitization effect of chitooligosaccharides (COS) on human colon cancer cell line SW480.
METHODS
SW480 cells were treated with 0, 1.0, 2.0, 3.0, 4.0 and 5.0 mg/mL of COS for 48 h. CCK-8 assay was employed to obtain the cell survival ratio of SW480 cells, and the anti-proliferation curve was observed with the inhibition ratio of COS on SW480 cells. The RAY + COS group was treated with 1.0 mg/mL of COS for 48 h, while both the RAY and RAY+COS groups were exposed to X-ray at 0, 1, 2, 4, 6 and 8 Gy, respectively. Clonogenic assay was used to analyze cell viability in the two groups at 10 d after treatment, and a cell survival curve was used to analyze the sensitization ratio of COS. The RAY group was exposed to X-ray at 6 Gy, while the RAY+COS group was treated with 1.0 mg/mL of COS for 48 h in advance and exposed to X-ray at 6 Gy. Flow cytometry was employed to detect cell cycle and apoptosis rate in the non-treatment group, as well as in the RAY and RAY + COS groups after 24 h of treatment.
RESULTS
COS inhibited the proliferation of SW480 cells, and the inhibition rate positively correlated with the concentration of COS (P < 0.01). Cell viability decreased as radiation dose increased in the RAY and RAY+COS groups (P < 0.01). Cell viabilities in the RAY+COS group were lower than in the RAY group at all doses of X-ray exposure (P < 0.01), and the sensitization ratio of COS on SW480 cells was 1.39. Compared with the non-treatment group, there was a significant increase in apoptosis rate in both the RAY and RAY + COS groups; while the apoptosis rate in the RAY+COS group was significantly higher than in the RAY group (P < 0.01). In comparing these three groups, the percentage of G2/M phase in both the RAY and RAY + COS groups significantly increased, and the percentage of the S phase and G0/G1 phase was downregulated. Furthermore, the percentage in the G2/M phase was higher, and the percentage in the S phase and G0/G1 phase was lower in the RAY + COS group vs RAY group (P < 0.01).
CONCLUSION
COS can inhibit the proliferation of SW480 cells and enhance the radiosensitization of SW480 cells, inducing apoptosis and G2/M phase arrest.
目的
研究壳寡糖（COS）对人结肠癌细胞系SW480的抗增殖及放射增敏作用。
方法
用0、1.0、2.0、3.0、4.0和5.0 mg/mL的COS处理SW480细胞48小时。采用CCK-8法检测SW480细胞的存活率，观察COS对SW480细胞的抑制率绘制抗增殖曲线。RAY + COS组用1.0 mg/mL的COS处理48小时，而RAY组和RAY+COS组分别接受0、1、2、4、6和8 Gy的X射线照射。采用克隆形成试验分析处理后10天两组细胞的活力，用细胞存活曲线分析COS的增敏率。RAY组接受6 Gy的X射线照射，而RAY+COS组预先用1.0 mg/mL的COS处理48小时后再接受6 Gy的X射线照射。采用流式细胞术检测未处理组以及处理24小时后的RAY组和RAY + COS组的细胞周期和凋亡率。
结果
COS抑制SW480细胞的增殖，抑制率与COS浓度呈正相关（P < 0.01）。RAY组和RAY+COS组中细胞活力随辐射剂量增加而降低（P < 0.01）。在所有X射线照射剂量下，RAY+COS组的细胞活力均低于RAY组（P < 0.01），COS对SW480细胞的增敏率为1.39。与未处理组相比，RAY组和RAY + COS组的凋亡率均显著增加；而RAY+COS组的凋亡率显著高于RAY组（P < 0.01）。比较这三组，RAY组和RAY + COS组中G2/M期的百分比均显著增加，S期和G0/G1期的百分比下调。此外，RAY + COS组中G2/M期的百分比高于RAY组，S期和G0/G1期的百分比低于RAY组（P < 0.01）。
结论
COS可抑制SW480细胞的增殖并增强其放射敏感性，诱导细胞凋亡和G2/M期阻滞。
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