Bakhshaie Mehdi, Khosravi Solmaz, Azadi Pejman, Bagheri Hedayat, van Tuyl Jaap M
Department of Horticultural Sciences, Faculty of Agriculture, University of Tehran, P.O. Box 31587-11167, Karaj, Iran.
Department of Genetic Engineering, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31535-1897, Karaj, Iran.
Plant Cell Rep. 2016 Sep;35(9):1799-826. doi: 10.1007/s00299-016-2017-8. Epub 2016 Jun 18.
Modern powerful techniques in plant biotechnology have been developed in lilies (Lilium spp., Liliaceae) to propagate, improve and make new phenotypes. Reliable in vitro culture methods are available to multiply lilies rapidly and shorten breeding programs. Lilium is also an ideal model plant to study in vitro pollination and embryo rescue methods. Although lilies are recalcitrant to genetic manipulation, superior genotypes are developed with improved flower colour and form, disease resistance and year round forcing ability. Different DNA molecular markers have been developed for rapid indirect selection, genetic diversity evaluation, mutation detection and construction of Lilium linkage map. Some disease resistance-QTLs are already mapped on the Lilium linkage map. This review presents latest information on in vitro propagation, genetic engineering and molecular advances made in lily.
现代强大的植物生物技术已在百合(百合属,百合科)中得到发展,用于繁殖、改良和创造新的表型。可靠的离体培养方法可用于快速繁殖百合并缩短育种计划。百合也是研究离体授粉和胚胎拯救方法的理想模式植物。尽管百合对基因操作具有顽拗性,但仍培育出了具有改良花色和花型、抗病性和全年促成栽培能力的优良基因型。已开发出不同的DNA分子标记,用于快速间接选择、遗传多样性评估、突变检测和构建百合连锁图谱。一些抗病性QTL已定位在百合连锁图谱上。本文综述了百合离体繁殖、基因工程和分子研究方面的最新信息。