酵母双杂交筛选确定WDR77为TSC22D2的一个新的相互作用蛋白。
Yeast two-hybrid screening identified WDR77 as a novel interacting partner of TSC22D2.
作者信息
Li Qiao, Chen Pan, Zeng Zhaoyang, Liang Fang, Song Yali, Xiong Fang, Li Xiayu, Gong Zhaojian, Zhou Ming, Xiang Bo, Peng Cong, Li Xiaoling, Chen Xiang, Li Guiyuan, Xiong Wei
机构信息
The Key Laboratory of Carcinogenesis and Cancer Invasion of the Chinese Ministry of Education, Xiangya Hospital, Central South University, Changsha, Hunan, China.
The Key Laboratory of Carcinogenesis of the Chinese Ministry of Health, Cancer Research Institute, Central South University, Changsha, Hunan, China.
出版信息
Tumour Biol. 2016 Sep;37(9):12503-12512. doi: 10.1007/s13277-016-5113-z. Epub 2016 Jun 23.
Transforming growth factor β-stimulated clone 22 domain family, member 2 (TSC22D2), a member of the TSC22D family, has been implicated as a tumor-associated gene, but its function remains unknown. To further explore its biological role, yeast two-hybrid screening combined with multiple bioinformatics tools was used to identify 44 potential interacting partners of the TSC22D2 protein that were mainly involved in gene transcription, cellular metabolism, and cell cycle regulation. The protein WD repeat domain 77 (WDR77) was selected for further validation due to its function in the cell cycle and tumor development, as well as its high detection frequency in the yeast two-hybrid assay. Immunoprecipitation and immunofluorescence experiments confirmed an interaction between the TSC22D2 and WDR77 proteins. Our work greatly expands the putative protein interaction network of TSC22D2 and provides deeper insight into the biological functions of the TSC22D2 and WDR77 proteins.
转化生长因子β刺激克隆22结构域家族成员2（TSC22D2）是TSC22D家族的一员，已被认为是一种肿瘤相关基因，但其功能仍不清楚。为了进一步探索其生物学作用，采用酵母双杂交筛选结合多种生物信息学工具，鉴定出44个TSC22D2蛋白的潜在相互作用伙伴，这些伙伴主要参与基因转录、细胞代谢和细胞周期调控。由于WD重复结构域77（WDR77）蛋白在细胞周期和肿瘤发展中的作用以及在酵母双杂交试验中的高检测频率，因此选择对其进行进一步验证。免疫沉淀和免疫荧光实验证实了TSC22D2和WDR77蛋白之间存在相互作用。我们的工作极大地扩展了TSC22D2假定的蛋白质相互作用网络，并为深入了解TSC22D2和WDR77蛋白的生物学功能提供了依据。
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