Derivation of HVR1, HVR2 and HVR3 human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis (PGD) for monogenic disorder.
作者信息
Hmadcha Abdelkrim, Aguilera Yolanda, Lozano-Arana Maria Dolores, Mellado Nuria, Sánchez Javier, Moya Cristina, Sánchez-Palazón Luis, Palacios Jose, Antiñolo Guillermo, Soria Bernat
机构信息
Department of Stem Cells, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Seville 41092, Spain; Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólica asociada (CIBERDEM), Madrid 28029, Spain.
Department of Stem Cells, Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER), Seville 41092, Spain.
出版信息
Stem Cell Res. 2016 May;16(3):635-9. doi: 10.1016/j.scr.2016.03.012. Epub 2016 Mar 31.
From 106 human blastocyts donate for research after in vitro fertilization (IVF) and preimplantation genetic diagnosis (PGD) for monogenetic disorder, 3 human embryonic stem cells (hESCs) HVR1, HVR2 and HVR3 were successfully derived. HVR1 was assumed to be genetically normal, HVR2 carrying Becker muscular dystrophy and HVR3 Hemophilia B. Despite the translocation t(9;15)(q34.3;q14) detected in HVR2, all the 3 cell lines were characterised in vitro and in vivo as normal hESCs lines and were registered in the Spanish Stem Cell Bank.
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