Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Department of Food Science and Technology, Kreutzwaldi 62, EE51014 Tartu, Estonia.
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Department of Food Science and Technology, Kreutzwaldi 62, EE51014 Tartu, Estonia.
Food Chem. 2016 Dec 1;212:117-22. doi: 10.1016/j.foodchem.2016.05.103. Epub 2016 May 17.
Bovine colostrum is potentially valuable source of essential fatty acids (FAs), but so far only few studies have made the effort to estimate FA composition of this potential resource. The aim of current research was to fill this gap with selecting and validating an accurate procedure for the analysis of the composition of the FAs in bovine colostrum. We used colostrum samples of Holstein-Friesian cattle from Märja experimental farm as a test material. The validated method includes derivatization, in which FAs are sent through esterification with the acidic catalyst boron trifluoride. Formed methyl esters of fatty acids (FAMEs) were analysed using GC-FID. The obtained LOD and the LOQ of FAMEs were 0.11-0.68 and 0.37-2.27ppm, respectively. The analysis of fortified samples showed very good and similar recoveries, indicating that the method proposed here can be routinely used for determination and investigation of the fatty acids in dairy products.
牛初乳是潜在的有价值的必需脂肪酸（FAs）来源，但迄今为止，只有少数研究努力估计这种潜在资源的 FA 组成。本研究旨在通过选择和验证一种准确的方法来分析牛初乳中 FA 的组成来填补这一空白。我们使用 Märja 实验农场的荷斯坦-弗里森牛的初乳样本作为测试材料。经验证的方法包括衍生化，其中 FA 通过与酸性催化剂三氟化硼的酯化反应转化为甲酯。形成的脂肪酸甲酯（FAMEs）使用 GC-FID 进行分析。FAMEs 的获得的检测限和定量限分别为 0.11-0.68 和 0.37-2.27ppm。加标样品的分析表明回收率非常好且相似，表明这里提出的方法可用于常规测定和研究乳制品中的脂肪酸。
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