短途运输对马气管细菌含量及下呼吸道细胞学的影响。

Influence of short distance transportation on tracheal bacterial content and lower airway cytology in horses.

作者信息

Allano Marion, Labrecque Olivia, Rodriguez Batista Edisleidy, Beauchamp Guy, Bédard Christian, Lavoie Jean-Pierre, Leclere Mathilde

机构信息

Faculté de Médecine Vétérinaire, Université de Montréal, 3200 Sicotte, Saint-Hyacinthe, Québec J2S 7C6, Canada.

Laboratoire d'épidemiosurveillance animale du Québec, Saint-Hyacinthe, Québec J2S 7C6, Canada.

出版信息

Vet J. 2016 Aug;214:47-9. doi: 10.1016/j.tvjl.2016.02.009. Epub 2016 Feb 20.

DOI:10.1016/j.tvjl.2016.02.009

PMID:27387726

Abstract

The aim of this study was to determine the effects of short distance transportation on airway mucus, cytology and bacterial culture to identify potential biases in the diagnosis of airway diseases in referral centres. Eight healthy adult horses were studied using a prospective cross-over design. Mucus scores, tracheal wash (cytology, bacterial culture) and bronchoalveolar lavage fluid (BALF; cytology) were obtained while stabled and following 2.5 h transportation (with and without hay). Neutrophil counts, percentages and BALF neutrophilia frequency increased following transport without hay (P <0.05). No effect was observed on tracheal cytology and bacterial count (P > 0.05). BALF neutrophilia could develop solely as a result of transportation or due to interactions between repeated transports, ambient temperature, head position or other environmental factors.

摘要

本研究的目的是确定短距离运输对气道黏液、细胞学和细菌培养的影响，以识别转诊中心气道疾病诊断中的潜在偏差。使用前瞻性交叉设计对8匹健康成年马进行了研究。在马匹厩养时以及在运输2.5小时后（有干草和无干草两种情况），获取黏液评分、气管冲洗液（细胞学、细菌培养）和支气管肺泡灌洗液（BALF；细胞学）。在无干草运输后，中性粒细胞计数、百分比和BALF中性粒细胞增多频率增加（P<0.05）。未观察到对气管细胞学和细菌计数有影响（P>0.05）。BALF中性粒细胞增多可能仅由运输导致，也可能是由于重复运输、环境温度、头部位置或其他环境因素之间的相互作用所致。