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死后微生物学分析中的细菌易位与样本污染

Bacterial Translocation and Sample Contamination in Postmortem Microbiological Analyses.

作者信息

Palmiere Cristian, Egger Coraline, Prod'Hom Guy, Greub Gilbert

机构信息

University Centre of Legal Medicine, University Hospital Center and University of Lausanne, Lausanne, Switzerland.

Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, Switzerland.

出版信息

J Forensic Sci. 2016 Mar;61(2):367-374. doi: 10.1111/1556-4029.12991. Epub 2015 Dec 31.

Abstract

The diagnostic value of postmortem bacteriology has been discussed controversially for decades. In the study herein, contamination during sampling procedures and postmortem translocation were investigated to interpret postmortem microbiology results. One hundred medicolegal autopsy cases in total were included. Radiology, histology, bacteriology, and biochemistry were performed in all cases. Based on all investigation findings, 4 groups of cases were identified: death unrelated to infection, true infections, false positive (contamination during sampling procedures, postmortem translocation and mixed situations), and undetermined. The results of this study indicate that postmortem bacteriology provides useful data supporting infection-related deaths, especially when potentially significant observations are accompanied by consistent autopsy, histology, and biochemistry. Result interpretation requires careful evaluation of number and type of isolated microorganisms.

摘要

几十年来,尸检细菌学的诊断价值一直存在争议。在本研究中,对采样过程中的污染和死后细菌移位进行了调查,以解释尸检微生物学结果。总共纳入了100例法医学尸检病例。所有病例均进行了放射学、组织学、细菌学和生物化学检查。根据所有调查结果,确定了4组病例:与感染无关的死亡、真正的感染、假阳性(采样过程中的污染、死后细菌移位和混合情况)以及无法确定的情况。本研究结果表明,尸检细菌学提供了支持与感染相关死亡的有用数据,特别是当潜在的重要观察结果伴有一致的尸检、组织学和生物化学检查时。结果解释需要仔细评估分离出的微生物的数量和类型。

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