关于未经授权的转基因番木瓜品系PRSV-YK实时聚合酶链反应检测的实验室间验证数据。

Interlaboratory validation data on real-time polymerase chain reaction detection for unauthorized genetically modified papaya line PRSV-YK.

作者信息

Nakamura Kosuke, Kondo Kazunari, Akiyama Hiroshi, Ishigaki Takumi, Noguchi Akio, Katsumata Hiroshi, Takasaki Kazuto, Futo Satoshi, Sakata Kozue, Fukuda Nozomi, Mano Junichi, Kitta Kazumi, Tanaka Hidenori, Akashi Ryo, Nishimaki-Mogami Tomoko

机构信息

National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.

FASMAC CO., LTD., 5-1-3 Midorigaoka, Atsugi, Kanagawa 243-0041, Japan.

出版信息

Data Brief. 2016 Apr 1;7:1165-70. doi: 10.1016/j.dib.2016.03.095. eCollection 2016 Jun.

DOI:10.1016/j.dib.2016.03.095

PMID:27408919

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4927967/

Abstract

This article is referred to research article entitled "Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method" (Nakamura et al., 2016) [1]. Real-time polymerase chain reaction (PCR) detection method for unauthorized genetically modified (GM) papaya (Carica papaya L.) line PRSV-YK (PRSV-YK detection method) was developed using whole genome sequence data (DDBJ Sequenced Read Archive under accession No. PRJDB3976). Interlaboratory validation datasets for PRSV-YK detection method were provided. Data indicating homogeneity of samples prepared for interlaboratory validation were included. Specificity and sensitivity test data for PRSV-YK detection method were also provided.

摘要

本文引用了题为《用于开发特定检测方法的未鉴定转基因木瓜全基因组序列分析》（中村等人，2016年）[1]的研究文章。利用全基因组序列数据（登录号PRJDB3976下的DDBJ测序读段存档）开发了未经授权的转基因（GM）木瓜（番木瓜）品系PRSV-YK的实时聚合酶链反应（PCR）检测方法（PRSV-YK检测方法）。提供了PRSV-YK检测方法的实验室间验证数据集。包括了用于实验室间验证的样本同质性数据。还提供了PRSV-YK检测方法的特异性和灵敏度测试数据。