长链非编码RNA GAS5是黑色素瘤细胞转移表型的关键调节因子，并在体内抑制肿瘤生长。
LncRNA GAS5 is a critical regulator of metastasis phenotype of melanoma cells and inhibits tumor growth in vivo.
作者信息
Chen Long, Yang Huixin, Xiao Yanbin, Tang Xiaoxia, Li Yuqian, Han Qiaoqiao, Fu Junping, Yang Yuye, Zhu Yuechun
机构信息
Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming, Yunnan, People's Republic of China; PET/CT Center, Yunan Tumor Hospital, the Third Affiliated Hospital of Kunming Medical University, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming, Yunnan, People's Republic of China.
出版信息
Onco Targets Ther. 2016 Jul 4;9:4075-87. doi: 10.2147/OTT.S98203. eCollection 2016.
The present study intended to demonstrate the effects of long noncoding RNA growth arrest-specific transcript 5 (GAS5) on the migration and invasion of melanoma cells. We first detected the expression of GAS5 among four kinds of melanoma cell lines, followed by constructing GAS5-knocked down and overexpressed stable cells. Next, we evaluated the effects of GAS5 on cell migration and invasion using wound healing and gelatin zymography assays. Finally, melanoma cells with different GAS5 expression were injected into nude mice, and the tumor volumes were recorded and tumor tissues were analyzed after sacrificing the mice. This study systematically examined the function of GAS5 in mediating melanoma metastasis and revealed that GAS5 plays an anticancer role in melanoma via regulating gelatinase A and B, both in vitro and in vivo.
本研究旨在证明长链非编码RNA生长停滞特异性转录本5（GAS5）对黑色素瘤细胞迁移和侵袭的影响。我们首先检测了四种黑色素瘤细胞系中GAS5的表达，随后构建了GAS5敲低和过表达的稳定细胞。接下来，我们使用伤口愈合和明胶酶谱分析评估GAS5对细胞迁移和侵袭的影响。最后，将具有不同GAS5表达的黑色素瘤细胞注射到裸鼠体内，记录肿瘤体积，并在处死小鼠后分析肿瘤组织。本研究系统地研究了GAS5在介导黑色素瘤转移中的作用，并揭示GAS5在体外和体内通过调节明胶酶A和B在黑色素瘤中发挥抗癌作用。
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