拟南芥SUMO E3连接酶AtMMS21在细胞周期调控中解离E2Fa/DPa复合物。
The Arabidopsis SUMO E3 Ligase AtMMS21 Dissociates the E2Fa/DPa Complex in Cell Cycle Regulation.
作者信息
Liu Yiyang, Lai Jianbin, Yu Mengyuan, Wang Feige, Zhang Juanjuan, Jiang Jieming, Hu Huan, Wu Qian, Lu Guohui, Xu Panglian, Yang Chengwei
机构信息
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou 510631, China.
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou 510631, China
出版信息
Plant Cell. 2016 Sep;28(9):2225-2237. doi: 10.1105/tpc.16.00439. Epub 2016 Aug 4.
Development requires the proper execution and regulation of the cell cycle via precise, conserved mechanisms. Critically, the E2F/DP complex controls the expression of essential genes during cell cycle transitions. Here, we discovered the molecular function of the SUMO E3 ligase METHYL METHANESULFONATE SENSITIVITY GENE21 (AtMMS21) in regulating the cell cycle via the E2Fa/DPa pathway. DPa was identified as an AtMMS21-interacting protein and AtMMS21 competes with E2Fa for interaction with DPa. Moreover, DPa is a substrate for SUMOylation mediated by AtMMS21, and this SUMOylation enhances the dissociation of the E2Fa/DPa complex. AtMMS21 also affects the subcellular localization of E2Fa/DPa. The E2Fa/DPa target genes are upregulated in the root of and mutants showed increased endoreplication. Overexpression of affected the root development of , and overexpression of completely recovered the abnormal phenotypes of plants. Our results suggest that AtMMS21 dissociates the E2Fa/DPa complex via competition and SUMOylation in the regulation of plant cell cycle.
发育需要通过精确、保守的机制正确执行和调控细胞周期。至关重要的是，E2F/DP复合物在细胞周期转变过程中控制必需基因的表达。在此，我们发现了SUMO E3连接酶甲磺酸甲酯敏感性基因21（AtMMS21）通过E2Fa/DPa途径调控细胞周期的分子功能。DPa被鉴定为与AtMMS21相互作用的蛋白，且AtMMS21与E2Fa竞争与DPa的相互作用。此外，DPa是AtMMS21介导的SUMO化修饰的底物，这种SUMO化修饰增强了E2Fa/DPa复合物的解离。AtMMS21还影响E2Fa/DPa的亚细胞定位。E2Fa/DPa靶基因在[具体植物名称]根中上调，[具体植物名称]和[具体植物名称]突变体显示核内复制增加。AtMMS21过表达影响[具体植物名称]的根发育，而AtMMS21过表达完全恢复了[具体植物名称]植物的异常表型。我们的结果表明，AtMMS21在植物细胞周期调控中通过竞争和SUMO化修饰使E2Fa/DPa复合物解离。
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