土耳其伊兹密尔省与溶血尿毒综合征相关的肠致病性大肠杆菌和产志贺毒素大肠杆菌的调查。
Investigation of enteropathogenic Escherichia coli and Shiga toxin-producingEscherichia coli associated with hemolytic uremic syndrome in İzmir Province, Turkey.
机构信息
Department of Biology, Basic and Industrial Microbiology Section, Faculty of Science, Ege University, İzmir, Turkey.
Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Ege University, İzmir, Turkey.
出版信息
Turk J Med Sci. 2016 Apr 19;46(3):733-41. doi: 10.3906/sag-1501-60.
BACKGROUND/AIM: The purpose of this study was to investigate Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic Escherichia coli (EPEC) strains originating from diarrheagenic patients.
MATERIALS AND METHODS
A total of 102 patients with diarrhea between October 2012 and January 2013 were enrolled in this study. Multiplex and standard polymerase chain reactions were performed to detect and distinguish STEC and EPEC strains. O serotyping of EPEC was carried out by monovalent antisera. The O and H serotyping of STEC strains was performed at the Refik Saydam Institute, Ankara.
RESULTS
A total of 5 (3.42%) strains were identified as STEC, and 3 strains (2.05%) were atypical EPEC. One of the STEC serotypes was O157:H7 carrying VT1, Stx1A, and escv genes. The other STEC strain was identified as O174:H21, which is associated with hemolytic uremic syndrome and consists of VT2 and Stx2A genes. One of the EPEC and three of the STEC serotypes were nontypeable. The serotypes of the atypical EPEC strains were identified as O114 and O26.
CONCLUSION
To the best of our knowledge, this is the first report of O174:H21 from the İzmir region that was shown to be a Shiga toxin-producing non-O157 serotype of STEC.
背景/目的：本研究旨在调查源自腹泻患者的产志贺毒素大肠杆菌（STEC）和肠致病性大肠杆菌（EPEC）菌株。
材料和方法
本研究共纳入了 2012 年 10 月至 2013 年 1 月期间的 102 例腹泻患者。采用多重和标准聚合酶链反应来检测和区分 STEC 和 EPEC 菌株。EPEC 的 O 血清型分型通过单价抗血清进行。STEC 菌株的 O 和 H 血清型分型在安卡拉的 Refik Saydam 研究所进行。
结果
共鉴定出 5 株（3.42%）为 STEC，3 株（2.05%）为非典型 EPEC。其中一种 STEC 血清型为 O157：H7，携带 VT1、Stx1A 和 escv 基因。另一种 STEC 菌株被鉴定为 O174：H21，与溶血尿毒综合征相关，包含 VT2 和 Stx2A 基因。EPEC 和 3 株 STEC 血清型中有 1 种不可分型。非典型 EPEC 菌株的血清型鉴定为 O114 和 O26。
结论
据我们所知，这是首次报道伊兹密尔地区的 O174：H21 是产志贺毒素的非 O157 型 STEC。
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