慢性移植肾肾病中移植物与脾脏之间Tfh细胞反应的差异
Differences in Tfh Cell Response Between the Graft and Spleen With Chronic Allograft Nephropathy.
作者信息
Shi Jian, Xu Xianlin, Luo Fengbao, Shi Qianqian, He Xiaozhou, Xia Ying
出版信息
Cell Transplant. 2017 Jan 24;26(1):95-102. doi: 10.3727/096368916X692816. Epub 2016 Aug 12.
The aim of this study was to investigate follicular helper T (Tfh) cell response and its difference between renal graft and spleen in a rat renal transplantation model undergoing chronic allograft nephropathy (CAN). Orthotopical kidney transplantations were performed on Fischer (F344) rats and transplanted to Lewis rats, using syngeneic Lewis-Lewis grafts as controls. Tissue samples were collected at 8 weeks posttransplantation. The status of Tfh cell response was assessed by measuring the levels of transcription factor B-cell lymphoma 6 (Bcl-6), interleukin-21 (IL-21), chemokine receptor type 5 (CXCR5), and B-cell-activating factor belonging to the TNF family (BAFF). Tfh cell response was upregulated in both renal graft and spleen of the CAN group compared to the control group. However, Tfh cell response of the spleen was weaker than that of the graft, which was possibly related to the upregulation of splenic Treg activation. Also, the difference between two tissues was partially associated with the different expressions of tristetraprolin (TTP)/IL-10. Our data help improve our understanding of the role of Tfh cell response in the body with CAN and may provide a valuable clue for better treatment of CAN.
本研究旨在探讨在经历慢性移植肾肾病（CAN）的大鼠肾移植模型中，滤泡辅助性T（Tfh）细胞反应及其在肾移植组织与脾脏之间的差异。将Fischer（F344）大鼠进行原位肾移植，并移植到Lewis大鼠体内，以同基因Lewis-Lewis移植作为对照。在移植后8周收集组织样本。通过测量转录因子B细胞淋巴瘤6（Bcl-6）、白细胞介素-21（IL-21）、趋化因子受体5型（CXCR5）以及肿瘤坏死因子家族的B细胞激活因子（BAFF）的水平，评估Tfh细胞反应的状态。与对照组相比，CAN组的肾移植组织和脾脏中的Tfh细胞反应均上调。然而，脾脏中的Tfh细胞反应弱于移植组织，这可能与脾脏中调节性T细胞（Treg）激活的上调有关。此外，两个组织之间的差异部分与锌指蛋白36（TTP）/白细胞介素-10的不同表达有关。我们的数据有助于增进我们对Tfh细胞反应在CAN机体中的作用的理解，并可能为更好地治疗CAN提供有价值的线索。
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