Guo Jian-Xin, Zhou Ya-Hong, Pan Jing-Xin, Guo Xi-Zhe, Wu Shi-Xin, Huang Yue-Qin
Department of Hematology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China.
Department of Hemodialysis, The Second Affiliated Hospital of Fujian Medical University, Quanzhou 362000, Fujian Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2016 Aug;24(4):1066-70. doi: 10.7534/j.issn.1009-2137.2016.04.020.
To explore the action mechanism of chlorambucil against mantle cell lymphoma cell line Jeko-1.
The effect of chlorambucil on Jeko-1 cell proliferation was measured by MTT method. The effect of chlorambucil on the apoptosis of Jeko-1 cell was detected by Hoechst staining and Annexin V-FITC dual staining. The activation of PI3K/AKT signaling pathway and the expression of BAX, BCL-2, procaspase 3, procaspase 8 and procaspase 9 were detected by Western blot.
0, 5, 10, 20 µmol/L chlorambucil could inhibit Jeko-1 cell proliferation at 24, 48, 72 h in a time- and dose-dependent manner. Chlorambucil of 0, 5, 10, 20 µmol/L increased the apoptotic rate of Jeko-1 cells, upregulated the expression of BAX, procaspase 3, procaspase 8, procaspase 9 and PI3K, increased the phosphorylation of AKT and down-regulated the expression of BCL-2.
The chlorambucil can induce the apoptosis of mantle cell lymphoma Jeko-1 cells via blocking PI3K/AKT signaling pathway.
探讨苯丁酸氮芥对套细胞淋巴瘤细胞系Jeko-1的作用机制。
采用MTT法检测苯丁酸氮芥对Jeko-1细胞增殖的影响。采用Hoechst染色和Annexin V-FITC双染法检测苯丁酸氮芥对Jeko-1细胞凋亡的影响。采用蛋白质免疫印迹法检测PI3K/AKT信号通路的激活情况以及BAX、BCL-2、procaspase 3、procaspase 8和procaspase 9的表达。
0、5、10、20 μmol/L苯丁酸氮芥在24、48、72 h可呈时间和剂量依赖性抑制Jeko-1细胞增殖。0、5、10、20 μmol/L苯丁酸氮芥可增加Jeko-1细胞凋亡率，上调BAX、procaspase 3、procaspase 8、procaspase 9和PI3K的表达，增加AKT磷酸化水平，下调BCL-2表达。
苯丁酸氮芥可通过阻断PI3K/AKT信号通路诱导套细胞淋巴瘤Jeko-1细胞凋亡。
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