肉桂链霉菌特定阻断突变体的筛选：26-脱氧莫能菌素A的分离与合成

Selection of a specifically blocked mutant of Streptomyces cinnamonensis: isolation and synthesis of 26-deoxymonensin A.

作者信息

Ashworth D M, Holmes D S, Robinson J A, Oikawa H, Cane D E

机构信息

Chemistry Department, The University, Southampton, UK.

出版信息

J Antibiot (Tokyo). 1989 Jul;42(7):1088-99. doi: 10.7164/antibiotics.42.1088.

DOI:10.7164/antibiotics.42.1088

PMID:2753815

Abstract

Streptomyces cinnamonensis produces the polyether ionophore antibiotic monensin A. Following a single round of mutagenesis by UV light, a derivative of this strain has been isolated, which secretes a new metabolite identified as 26-deoxymonensin A (3). The structural elucidation of the new metabolite followed from a spectroscopic analysis, and its identity was proven conclusively following a comparison to 26-deoxymonensin A (3) obtained synthetically from monensin A. The preparation of labelled forms of 3 is described, together with incorporation experiments using the parent strain of S. cinnamonensis. Only very low levels of incorporation of 3 into monensin A were observed.

摘要

肉桂链霉菌产生聚醚离子载体抗生素莫能菌素A。在通过紫外线进行一轮诱变后，已分离出该菌株的一种衍生物，其分泌一种新的代谢产物，鉴定为26-脱氧莫能菌素A（3）。通过光谱分析对新代谢产物进行了结构解析，通过与从莫能菌素A合成得到的26-脱氧莫能菌素A（3）进行比较，最终证实了其身份。描述了3的标记形式的制备以及使用肉桂链霉菌亲本菌株的掺入实验。仅观察到3掺入莫能菌素A的水平非常低。