Fernandes João S, Gentile Piergiorgio, Martins Margarida, Neves Nuno M, Miller Cheryl, Crawford Aileen, Pires Ricardo A, Hatton Paul, Reis Rui L
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
Centre for Biomaterials and Tissue Engineering, School of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield S10 2TA, United Kingdom.
Acta Biomater. 2016 Oct 15;44:168-77. doi: 10.1016/j.actbio.2016.08.042. Epub 2016 Aug 21.
Herein, for the first time, we combined poly-l-lactic acid (PLLA) with a strontium borosilicate bioactive glass (BBG-Sr) using electrospinning to fabricate a composite bioactive PLLA membrane loaded with 10% (w/w) of BBG-Sr glass particles (PLLA-BBG-Sr). The composites were characterised by scanning electron microscopy (SEM) and microcomputer tomography (μ-CT), and the results showed that we successfully fabricated smooth and uniform fibres (1-3μm in width) with a homogeneous distribution of BBG-Sr microparticles (<45μm). Degradation studies (in phosphate buffered saline) demonstrated that the incorporation of BBG-Sr glass particles into the PLLA membranes increased their degradability and water uptake with a continuous release of cations. The addition of BBG-Sr glass particles enhanced the membrane's mechanical properties (69% higher Young modulus and 36% higher tensile strength). Furthermore, cellular in vitro evaluation using bone marrow-derived mesenchymal stem cells (BM-MSCs) demonstrated that PLLA-BBG-Sr membranes promoted the osteogenic differentiation of the cells as demonstrated by increased alkaline phosphatase activity and up-regulated osteogenic gene expression (Alpl, Sp7 and Bglap) in relation to PLLA alone. These results strongly suggest that the composite PLLA membranes reinforced with the BBG-Sr glass particles have potential as an effective biomaterial capable of promoting bone regeneration.
PLLA membranes were reinforced with 10% (w/w) of strontium-bioactive borosilicate glass microparticles, and their capacity to induce the osteogenic differentiation of bone marrow mesenchymal stem cells (BM-MSCs) was evaluated. These membranes presented an increased: degradability, water uptake, Young modulus and tensile strength. We also demonstrated that these membranes are non-cytotoxic and promote the attachment of BM-MSCs. The addition of the glass microparticles into the PLLA membranes promoted the increase of ALP activity (under osteogenic conditions), as well as the BM-MSCs osteogenic differentiation as shown by the upregulation of Alpl, Sp7 and Bglap gene expression. Overall, we demonstrated that the reinforcement of PLLA with glass microparticles results in a biomaterial with the appropriate properties for the regeneration of bone tissue.
在此，我们首次使用静电纺丝技术将聚左旋乳酸（PLLA）与硼硅酸锶生物活性玻璃（BBG-Sr）相结合，制备了负载10%（w/w）BBG-Sr玻璃颗粒的复合生物活性PLLA膜（PLLA-BBG-Sr）。通过扫描电子显微镜（SEM）和微型计算机断层扫描（μ-CT）对复合材料进行了表征，结果表明我们成功制备了宽度为1-3μm的光滑均匀纤维，且BBG-Sr微粒（<45μm）分布均匀。降解研究（在磷酸盐缓冲盐溶液中）表明，将BBG-Sr玻璃颗粒掺入PLLA膜中可提高其降解性和吸水性，并持续释放阳离子。添加BBG-Sr玻璃颗粒增强了膜的机械性能（杨氏模量提高69%，拉伸强度提高36%）。此外，使用骨髓间充质干细胞（BM-MSCs）进行的细胞体外评估表明，与单独的PLLA相比，PLLA-BBG-Sr膜通过增加碱性磷酸酶活性和上调成骨基因表达（Alpl、Sp7和Bglap）促进了细胞的成骨分化。这些结果有力地表明，用BBG-Sr玻璃颗粒增强的复合PLLA膜具有作为促进骨再生的有效生物材料的潜力。
用10%（w/w）的锶生物活性硼硅酸盐玻璃微粒增强PLLA膜，并评估其诱导骨髓间充质干细胞（BM-MSCs）成骨分化的能力。这些膜的降解性、吸水性、杨氏模量和拉伸强度均有所提高。我们还证明这些膜无细胞毒性并促进BM-MSCs的附着。在PLLA膜中添加玻璃微粒促进了碱性磷酸酶活性（在成骨条件下）的增加，以及Alpl、Sp7和Bglap基因表达上调所显示的BM-MSCs成骨分化。总体而言，我们证明用玻璃微粒增强PLLA可得到具有适合骨组织再生性能的生物材料。
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