由包括哈氏假丝酵母复合体在内的多重耐药酵母产生的不同类别的水解酶。
Different classes of hydrolytic enzymes produced by multidrug-resistant yeasts comprising the Candida haemulonii complex.
作者信息
Ramos Lívia S, Branquinha Marta H, Santos André L S
机构信息
Laboratório de Investigação de Peptidases, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Laboratório de Investigação de Peptidases, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
出版信息
Med Mycol. 2017 Feb 1;55(2):228-232. doi: 10.1093/mmy/myw065. Epub 2016 Aug 23.
The production of enzymes in clinical isolates of Candida haemulonii (Ch, n = 5), Candida duobushaemulonii (Cd, n = 4) and Candida haemulonii var. vulnera (Chv, n = 3) was identified by agar plate. Aspartic protease, phytase, caseinolytic and hemolytic activities were detected in all the isolates. A distinct scenario was evidenced regarding the production of lipases. In this way, 80%, 50% and 100% of Ch, Cd and Chv, respectively, were phospholipase producers. Regarding esterase activity, 100%, 50% and 66.7% of Ch, Cd and Chv, respectively, were positive isolates. Esterase activity was significantly higher in isolates recovered from cutaneous candidiasis compared with those recovered from body fluids.
通过琼脂平板法鉴定了哈氏假丝酵母菌临床分离株（Ch，n = 5）、多布哈氏假丝酵母菌（Cd，n = 4）和哈氏假丝酵母菌脆弱变种（Chv，n = 3）中酶的产生情况。在所有分离株中均检测到天冬氨酸蛋白酶、植酸酶、酪蛋白分解活性和溶血活性。在脂肪酶产生方面呈现出不同的情况。这样，Ch、Cd和Chv分别有80%、50%和100%为磷脂酶产生菌。关于酯酶活性，Ch、Cd和Chv分别有100%、50%和66.7%的分离株呈阳性。与从体液中分离出的菌株相比，从皮肤念珠菌病中分离出的菌株酯酶活性显著更高。
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